货号：AB5152-200UL

品牌：Merck Millipore

规格：1EA

目录价：￥8283.37

市场价格：￥7040.86

会员价格：￥6626.70

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Calcium Channel Antibody, Voltage Gated α 1A | AB5152-200UL View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes Cav2.1 (alpha 1A subunit, P/Q-type of voltage-gated calcium channel, Cacna1a). Does not cross react with any other calcium channel proteins tested so far. View All» Immunogen: Purified peptide CNA1 from 865-881 of alpha 1A subunit of rat brain voltage-gated calcium channel (Starr, 1991) (Accession P54282) containing N-terminal lysine and tyrosine, conjugated to KLH with gluteraldehyde (Westenbroek et al. 1995). View All» Species Reactivity:

• Mouse

• Rat
  View All» Application Notes: All procedures that are going to receive a full-length protein should be performed at 4 °C, and the following protease inhibitor mixture should be used: pepstatin A (1 mg/mL), leupeptin (1 mg/mL), aprotinin (1 mg/mL), Pefabloc SC (0.2 mM), benzamidine (0.1 mg/mL), and calpain inhibitors I and II (8 mg/mL each). Western blot: 1:200 using ECL on rat brain membranes. Immunohistochemistry on rat brain sections.Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. Immunocytochemistry: 5 μg/mL (1:60). Recommend subsequent staining with a labeled second antibody. Western blot: 1.5 μg/mL (1:200) using ECL Dilutions should be made using a carrier protein such as BSA (1-3%) Optimal working dilutions must be determined by the end user. View All» Control: CONTROL ANTIGEN: Included free of charge with the antibody is 20 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 10 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user. View All» Presentation: Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 200 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 x g for 5 min). View All» Storage Conditions: Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution add glycerol (ASC gade or better at a ratio of 1:1 and maintain at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles. View All» UniProt Number: O00555 View All» Entrez Gene Number:
  • NM_000068.3

  • NM_023035.2
    View All» Gene Symbol:
    • CACNA1A

    • FHM

    • CAV2.1

    • CACNL1A4

    • SCA6

    • BI

    • HPCA

    • CACN3

    • MHP1

    • Cav2.1

    • CACH4

    • APCA

    • MHP

    • EA2
      View All» Alternate Names: P/Q-type View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to (CAG)n-repeats. Multiple transcript variants have been described, however, the full-length nature of not all is known. In one set of transcript variants, the (CAG)n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the (CAG)n-repeats which encode a polyglutamine tract. Expansion of the (CAG)n-repeats from the normal 4-16 to 21-28 in the coding region is associated with spinocerebellar ataxia 6. View All» UniProt Summary: FUNCTION: SwissProt: O00555 # Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1A gives rise to P and/or Q-type calcium currents. P/Q-type calcium channels belong to the high-voltage activated (HVA) group and are blocked by the funnel toxin (Ftx) and by the omega-agatoxin- IVA (omega-Aga-IVA). They are however insensitive to dihydropyridines (DHP), and omega-conotoxin-GVIA (omega-CTx-GVIA).SIZE: 2505 amino acids; 282365 Da SUBUNIT: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore- forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.TISSUE SPECIFICITY: Brain specific; mainly found in cerebellum, cerebral cortex, thalamus and hypothalamus. No expression in heart, kidney, liver or muscle. Purkinje cells contain predominantly P-type VSCC, the Q-type being a prominent calcium current in cerebellar granule cells.DOMAIN: SwissProt: O00555 Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.DISEASE: SwissProt: O00555 # Defects in CACNA1A are the cause of spinocerebellar ataxia 6 (SCA6) [MIM:183086]. Spinocerebellar ataxia is a clinically and genetically heterogeneous group of autosomal dominant cerebellar ataxias (ADCA). Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements. Spinocerebellar ataxia is caused by degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA6 is caused by expansion of a CAG repeat in the coding region of CACNA1A. There seems to be a correlation between the repeat number and earlier onset of the disorder. & Defects in CACNA1A are the cause of familial hemiplegic migraine (FHM) [MIM:141500]; also known as migraine familial hemiplegic 1 (MHP1). FHM, a rare autosomal dominant subtype of migraine with aura, is associated with ictal hemiparesis and, in some families, progressive cerebellar atrophy. & Defects in CACNA1A are the cause of episodic ataxia type 2 (EA-2) [MIM:108500]; also known as acetazolamide-responsive hereditary paroxysmal cerebellar ataxia (APCA). This autosomal dominant disorder is characterized by acetozolamide-responsive attacks of cerebellar ataxia and migraine-like symptoms, interictal nystagmus, and cerebellar atrophy.SIMILARITY: Belongs to the calcium channel alpha-1 subunit family. View All» Brand Family: Chemicon View All» Product Name: Anti-Calcium Channel Antibody, Voltage Gated α 1A | AB5152-200UL View All» Concentration: 0.75mg/ml(after reconstitution) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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