货号：AB3573

品牌：Merck Millipore

规格：1EA

目录价：￥6480.55

市场价格：￥5508.47

会员价格：￥5184.44

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-BCL-XL (pS62) Antibody | AB3573 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Human Bcl-xL. Mouse and rat Bcl-xL (100% conserved) have not been tested, but are expected to react. View All» Immunogen: The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Bcl-xL that contains serine 62. The sequence is conserved in mouse and rat. View All» Modifications: Phosphorylation View All» Background Information: Bcl-xL is a ~28 kDa member of Bcl-2 family of proteins and an important regulator of apoptosis. Bcl-xL forms heterodimers with BAX, BAK, and Bcl-2, and its overexpression in tumor cells confers resistance against chemotherapeutic drugs. Bcl-xL is phosphorylated on many sites including serine 62, a critical site for Bcl-xL response to microtubule-damaging drugs such as taxol and vinblastine. Phosphorylation of serine 62 (thought to be mediated by Jun N-terminal stress kinase (JNK) signaling) negatively regulates the anti-apoptotic function of Bcl-xL and controls the growth of neoplastic cells. View All» Species Reactivity: Human View All» Species Reactivity Note: Mouse and rat Bcl-xL (100% homologous) have not been tested, but are expected to react. View All» Application Notes: For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. View All» Control: SK-BR-3 cells treated with vinblastine. View All» Quality Assurance: Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bcl-xL. The final product is generated by affinity chromatography using a Bcl-xL-derived peptide that is phosphorylated at serine 62. View All» Presentation: Dulbecco's phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide. View All» Storage Conditions: Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient. View All» UniProt Number: Q92934 View All» Entrez Gene Number:

• NM_004322.2

• NM_032989.1
  View All» Gene Symbol:
  • BAD

  • BBC2

  • BBC6

  • BCL2L8
    View All» Alternate Names: Bcl-2-binding component 6; Bcl-XL/Bcl-2-associated death promoter; Bcl-2-like 8 protein; BAD; BBC6; BCL2L8 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. View All» UniProt Summary: FUNCTION: SwissProt: Q92934 # Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.SIZE: 168 amino acids; 18392 Da SUBUNIT: Forms heterodimers with the anti-apoptotic proteins, Bcl- X(L), Bcl-2 and Bcl-W. Also binds protein S100A10 (By similarity). The Ser-75/Ser-99 phosphorylated form binds 14-3-3 proteins (By similarity).SUBCELLULAR LOCATION: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm.TISSUE SPECIFICITY: Expressed in a wide variety of tissues.DOMAIN:SwissProt: Q92934 Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.PTM: Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro- apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation (By similarity).SIMILARITY: Belongs to the Bcl-2 family. View All» Brand Family: Chemicon View All» Product Name: Anti-BCL-XL (pS62) Antibody | AB3573 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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