货号：AB3080

品牌：Merck Millipore

规格：50Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Green Fluorescent Protein Antibody | AB3080 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB3080 is made against highly-purified native GFP from Aequorea victoria. It is reactive with GFP from both native and recombinant sources. While other commercial antibodies to GFP typically demonstrate strong reactivity with E. coli, the AB3080 GFP antibody has been purified by Protein A chromatography, followed by extensive adsorption against whole E. coli BL21 strain cells, giving them minimal cross-reactivity with E. coli. View All» Molecular Weight: GFP is 27 kDa. Dependent upon the molecular weight of the fusion protein being detected. View All» Immunogen: Highly purified native GFP from Aequorea victoria. View All» Background Information: The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. View All» Species Reactivity: Vertebrates View All» Application Notes: Western Blot: A previous lot of this antibody was used in western blot.ELISA: A 1:2000-1:10,000 dilution of a previous lot was used in Enzyme-linked Immunosorbent Assay.Note: While AB3080 has been adsorbed against E. coli proteins, most secondary antibodies have not. To reduce the likelihood of E. coli reactivity from a secondary antibody, we suggest the use of Millipore’s highly-purified secondary antibodies in indirect detection systems. Immunocytochemistry:Optimal working dilutions must be determined by end user. View All» Control:

• GFP fusion protein expressed in cells

• RENCX cells
  View All» Quality Assurance: Routinely evaluated by immunocytochemistry on RENCX cells.Immunocytochemistry Analysis: Confocal fluorescent analysis of RENCX constitutively expressed GFP using this lot of anti-GFP Rabbit pAB (Red). Green indicates the expressed GFP. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal IgG in 10 mM Tris buffer, pH 7.2 with 0.01% sodium azide. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P42212 View All» Gene Symbol:
  • GFP

  • eGFP
    View All» Alternate Names: GFP; eGFP View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunocytochemistry

    • Immunohistochemistry

    • Western Blotting
      View All» Brand Family: Chemicon View All» Product Name: Anti-Green Fluorescent Protein Antibody | AB3080 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Purified View All» Host: Rabbit View All»
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