金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Accession #:P51668 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:17 kDa Activity:Recombinant Human UbcH5a/UBE2D1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human UbcH5a/UBE2D1 concentration of 0.1-1 µM. Formulation:Supplied as a solution in HEPES, NaCl, Glycerol.See Certificate of Analysis for details. Molecule Information: UbcH5a/UBE2D1 Aliases: E2(17)KB1; UBC4/5; UBC5A; UBE2D1 Entrez Gene IDs: 7321 (Human); 216080 (Mouse); 361831 (Rat) Background: Ubch5a Ubiquitin-conjugating Enzyme H5a (UbcH5a), also known as Ubiquitin-conjugating Enzyme E2D 1 (UBE2D1), is a ubiquitously expressed protein that is related to Stimulator of Iron Transport (SFT). Human UbcH5a/UBE2D1 has a predicted molecular weight of 17 kDa and shares 89% and 88% amino acid (aa) sequence identity with the related family members UbcH5b and UbcH5c, respectively. Human UbcH5a/UBE2D1 shares 100% aa sequence identity with the mouse and rat orthologs. UbcH5a/UBE2D1 has a conserved E2 catalytic core domain that contains an active site cysteine residue, and it interacts with a variety of HECT and RING finger Ubiquitin ligases (E3) to mediate the ubiquitination of specific target proteins. UbcH5a/UBE2D1 interacts with the E3, E6-AP, to conjugate Ubiquitin to the tumor suppressor, p53. Additional protein targets of UbcH5a/UBE2D1 include c-Fos, RIP1, and HIF-1. Pathologically, UbcH5a/UBE2D1 is implicated in protein degradation during cancer and immune responses.