货号：AB2955

品牌：Merck Millipore

规格：EA

目录价：￥3828.00

市场价格：￥3253.80

会员价格：￥3062.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PIST Antibody | AB2955 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody recognizes PIST near the C-terminus. View All» Molecular Weight: ~ 55kDa Observed View All» Epitope: Near the C-terminus View All» Immunogen: KLH-conjugated linear peptide corresponding to mouse PIST near the C-terminus. View All» Background Information: PDZ domain protein interacting specifically with TC10 (PIST) is also known as Golgi-associated PDZ and coiled-coil motif-containing protein, a protein consisting of 462 amino acids. PIST has an important role in the trafficking of various intracellular proteins and degradation within the cytoplasm in association with the Golgi apparatus. It is necessary in frizzled protein transport and transporting the cystic fibrosis transmembrane conductance regulator (CTFR) to the plasma membrane. PIST has two N-terminal coiled-coil domains in addition to its C-terminal PDZ domain, and is ubiquitously expressed in tissue including synaptosomal and postsynaptic density fractions. It has been suggested that PIST may play a role in autophagy and also neurodegeneration. Overexpression of PIST is likely to be causal in CTFR intracellular retention leading to lysosomal degradation. The involvement of PIST within Chromosomal aberrations has been observed in glioblastoma multiform. View All» Species Reactivity:

• Mouse

• Human

• Rhesus Macaque

• Canine

• Ox
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. Predicted to react with rhesus macaque, canine, and ox based on 100% sequence homology. View All» Application Notes: Western Blot (SNAP ID) Analysis: 1 µg/mL of this antibody has been shown to detect PIST in 10 µg of mouse brain lysate.Immunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect PIST in normal human brain tissue. View All» Control: Mouse brain lysate View All» Quality Assurance: Evaluated by Western Blot in mouse brain lysate.Western Blot Analysis: 0.5 µg/mL of this antibody detected PIST in 10 µg of mouse brain lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q8BH60 View All» Entrez Gene Number: NP_444417 View All» Gene Symbol:
  • PIST

  • FIG

  • CAL

  • GOPC1
    View All» Alternate Names:
    • Golgi-associated PDZ and coiled-coil motif-containing protein

    • CFTR-associated ligand

    • PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10

    • PDZ protein interacting specifically with TC10
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» UniProt Summary: FUNCTION: Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes. SUBUNIT STRUCTURE: Homooligomer. Interacts with STX6 and GOLGA3 by similarity. Interacts with ACCN3, RHOQ, FZD5, FZD8, GRID2, BECN1, CSPG5, CFTR and CLCN3. May interact with CACNG2. SUBCELLULAR LOCATION: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Cell junction › synapse. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density. Cell projection › dendrite. Note: Enriched in synaptosomal and postsynaptic densities (PSD) fractions. Expressed in cell bodies and dendrites of Purkinje cells. Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides. TISSUE SPECIFICITY: Ubiquitously expressed (at protein level). Expressed in dorsal root glanglion (DRG), spinal chord and brain. Isoform 1 is preferentially expressed in whole brain (at protein level) and cerebellum. Expressed in spermatocytes and spermatides but not in Sertoli cells and spermatogonia. DEVELOPMENTAL STAGE: In the cerebellum, expression increases post-natally, following maturation of this tissue (at protein level). DOMAIN: The coiled-coil region probably mediates targeting to the Golgi, oligomerization and interaction with RHOQ. May also mediates association to membranes and interactions with GOLGA3 and STX6 (by similarity). The PDZ domain mediates interaction with ADRB1 by similarity. Mediates also interactions with FZD5, FZD8, ACCN3, GRID2, CFTR, CLCN3. DISRUPTION PHENOTYPE: Male mice are infertile with globozoospermia. Spermatozoa display a default in acrosome formation and are unable to activate oocytes. SEQUENCE SIMILARITIES: Contains 1 PDZ (DHR) domain. View All» Product Name: Anti-PIST View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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