金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Ala2 - Tyr249 Accession #:NP_058960 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain. Predicted Molecular Mass:29 kDa Activity:Recombinant Human PA28 Activator alpha Subunit is ideal for the activation of latent 20S Proteasome complexes. Reaction conditions will need to be optimized for each specific application. Initially, we recommend a 5-15-fold molar excess of Recombinant Human PA28 Activator alpha Subunit relative to 20S Proteasome. Formulation:Supplied as a solution in HEPES, NaCl, DTT and EDTA.See Certificate of Analysis for details. Molecule Information: PA28 Activator alpha Subunit Long Name: Proteasome (Prosome, Macropain) Activator Subunit 1 Aliases: 11S Regulator Complex Subunit alpha; IFI5111; PSME1; REG alpha Entrez Gene IDs: 5720 (Human); 19186 (Mouse); 29630 (Rat) Background: PA28 Activator alpha Subunit The Proteasome Activator 28 (PA28) Regulatory Complex, also known as the 11S Regulatory (REG) Complex, is a ring-shaped, multimeric, ATP-independent regulatory complex that can bind to one or both ends of the 20S Proteasome or 20S Immunoproteasome. The PA28 Regulatory Complex stimulates the proteasome to hydrolyze small peptides, but not ubiquitinated proteins, for the production of peptides for MHC class I antigen presentation. Three PA28 Regulatory Complex subunits, alpha, beta, and gamma, have been identified and can oligomerize to form the PA28 Regulatory Complex. While the expression of the PA28 alpha and beta subunits are induced by IFN-gamma, PA28 gamma is not significantly affected.

The human PA28 Activator alpha Subunit, also known as 11S REG alpha, is a 249 amino acid (aa) member of the PA28 family of regulatory proteins with a predicted molecular weight of 28 kDa. The mouse and rat orthologs share 94% and 95% aa sequence identity with the human protein, respectively. In vivo, PA28 alpha forms hetero-heptamers or -hexamers with PA28 beta. PA28 alpha can also form active homo-heptamers in vitro and can be used to activate the 20S Proteasome. In addition to regulating production of MHC class I antigens, PA28 alpha may protect against oxidative stress.