货号：AB2047

品牌：Merck Millipore

规格：100Ug

目录价：￥5315.00

市场价格：￥4517.75

会员价格：￥4252.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Fibronectin Antibody | AB2047 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Antibody reacts with bovine fibronectin, and demonstrates cross-reactivity of less than 0.1% with bovine collagens types I, III, IV by RIA at 1:10,000 dilution. View All» Immunogen: Fibronectin extracted and purified from bovine plasma. View All» Background Information: Fibronectin (FN) is an extracellular adhesion molecule and it is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells. Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates. FN also can serve to organize cellular interaction with the ECM by binding to different components of the extracellular matrix and to membrane-bound FN receptors on cell surfaces. The importance of fibronectin in cell migration events during embryogenesis has been documented in several contexts, e.g.: 1) mesodermal cell migration during gastrulation can be blocked by injection of Arg-Gly-Asp (RGD) tripeptides that block cellular FN receptors (integrins); 2) injection of anti-FN antibodies into chick embryos blocks migration of precardiac cells to the embryonic midline, and; 3) the patterns of FN deposition in developing vertebrate limbs determines the patterns of precartilage cell adhesion to the ECM, thereby specifying limb-specific patterns of chondrogenesis. {D. Marcey, http://www.clunet.edu/BioDev/omm/fibro/frames/fibrotxt.htm}. View All» Species Reactivity: Bovine View All» Application Notes: Immunohistochemistry: 1:80 dilution for immunofluorescent staining of fresh frozen bovine skin and liver tissues. Acetone or methyl-carnoy fixation is also reactive. Immunohistochemistry on paraffin embedded tissues requires light fixation in 2% PFA, 4% formalin (less than 90 minutes), acetone or methyl-carnoy fixation; traditional formalin fixation is not recommended. Antigen retrieval is HIER citrate buffer; detection is via enhanced enzymatic methods only. Immunoblotting: 1:1000 of 2% deoxycholate + 10% SDS, 6M urea extracted bovine cell cultures (Kinsella, 2000). Antibody demonstates the appropriate twin bands at ~220kDa. Radioimmunoassay ELISA Optimal working dilutions must be determined by end user. View All» Presentation: Protein G affinity purified IgG fraction at 1.0 mg/mL in liquid PBS (0.01M phosphate, 0.09M NaCl) pH 7.2 with no preservatives. View All» Storage Conditions: Maintain frozen at -20°C for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P02751 View All» Entrez Gene Number:

• NM_002026.2

• NM_054034.2

• NM_212474.1

• NM_212475.1

• NM_212476.1

• NM_212478.1

• NM_212482.1
  View All» Gene Symbol:
  • FN1

  • DKFZp686O13149

  • DKFZp686H0342

  • DKFZp686I1370

  • MSF

  • FN

  • CIG

  • DKFZp686F10164

  • LETS

  • FINC
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunofluorescence

    • Radioimmunoassay

    • Western Blotting

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. View All» UniProt Summary: FUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).SIZE: 2386 amino acids; 262607 Da SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.PTM: Sulfated.SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains. View All» Brand Family: Chemicon View All» Product Name: Anti-Fibronectin Antibody | AB2047 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rabbit View All»
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