货号：AB19167

品牌：Merck Millipore

规格：

目录价：￥4264.00

市场价格：￥3624.40

会员价格：￥3411.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MMP-2 Antibody, whole molecule | AB19167 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: AB19167 recognizes protein at 72 kDa, identified as matrix metalloproteinase 2. MMP-2 is also known as 72 kDa collagenase IV or gelatinase A. It is synthesized as a 631 amino acid proenzyme that is activated by cleavage of the first 80 amino acids. AB19167 shows no cross-reaction with (pro) and active forms of other MMPs. Cytoplasmic localization. View All» Molecular Weight: 72 kDa View All» Epitope: Whole molecule View All» Immunogen: A synthetic peptide from the second half of human MMP-2. View All» Background Information: MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer of the stromal cells. There is a co-operation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction. View All» Species Reactivity:

• Bovine

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Bovine and Rat. Expected to react with human and mouse based on sequence homology. Reactivity with other species has not been confirmed. View All» Application Notes: Immunohistochemistry (formalin/paraffin): 10-20 µg/mL of a previous lot was kept for 30 min at RT. No special pretreatment is required for staining formalin-fixed paraffin-embedded sections.Optimal working dilutions must be determined by the end user. View All» Control: Positive control: Conditioned, serum-free medium from TPA-treated human fetal lung (HFL-1) cells. Placenta or bladder, breast, or ovarian carcinomas. View All» Quality Assurance: Routinely evaluated by Western Blot on PC12 lysates.Western Blot Analysis: 1:500 dilution of this lot detected MMP-2 on 10 µg of PC12 lysates. View All» Purification Method: Protein A chromatography View All» Presentation: Purified rabbit polyclonal in buffer containing 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt. View All» UniProt Number: P08253 View All» Entrez Gene Number: NM_004530.2 View All» Gene Symbol:
  • CLG4

  • CLG4A

  • EC 3.4.24.24

  • MMP-2

  • MMP-II

  • MONA

  • TBE-1
    View All» Alternate Names:
    • 72 kDa gelatinase

    • 72kD type IV collagenase

    • Gelatinase A

    • Matrix metalloproteinase-2

    • collagenase type IV-A

    • matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

    • matrix metalloproteinase 2

    • matrix metalloproteinase 2 (gelatinase A, 72kD gelatinase, 72kD type IV collagenase)

    • matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)

    • matrix metalloproteinase-II

    • neutrophil gelatinase
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry (Paraffin)

      • Western Blotting
        View All» Entrez Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. View All» UniProt Summary: FUNCTION: SwissProt: P08253 # In addition to gelatin and collagens, it cleaves KiSS1 at a Gly- -Leu bond.COFACTOR: Binds 4 calcium ions per subunit. & Binds 2 zinc ions per subunit.SIZE: 660 amino acids; 73882 Da SUBUNIT: Ligand for integrin alpha-V/beta-3.TISSUE SPECIFICITY: Produced by normal skin fibroblasts.DOMAIN: SwissProt: P08253 The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.PTM: The propeptide is processed by MMP14 (MT-MMP1) and MMP16 (MT- MMP3).DISEASE: SwissProt: P08253 # Defects in MMP2 are the cause of multicentric osteolysis nodulosis and arthropathy (MONA) [MIM:605156]. Inherited osteolyses or 'vanishing bone' syndromes are rare disorders of unknown etiology characterized by destruction and resorption of affected bones. MONA is an autosomal recessive osteolysis with multicentric involvement characterized by carpal and tarsal resorption, crippling arthritic changes, marked osteoporosis, palmar and plantar subcutaneous nodules and distinctive facies. & Defects in MMP2 are the cause of Winchester syndrome [MIM:277950]. Winchester syndrome is an autosomal recessive osteolysis syndrome. Winchester syndrome is severe with generalized osteolysis and osteopenia. Subcutaneous nodules are usually absent. Winchester syndrome has been associated with a number of additional features including coarse face, corneal opacities, patches of thickened, hyperpigmented skin, hypertrichosis and gum hypertrophy. However, these features are not always present and have occasionally been observed in other osteolysis syndromes. The clinical and molecular findings suggest that Winchester syndrome and MONA are allelic disorders that form a continuous clinical spectrum.SIMILARITY: Belongs to the peptidase M10A family. & Contains 3 fibronectin type-II domains. & Contains 4 hemopexin-like domains. View All» Brand Family: Chemicon View All» Product Name: Anti-MMP-2 Antibody, whole molecule | AB19167 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Rabbit View All»
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