货号：AB1854

品牌：Merck Millipore

规格：100UL

目录价：￥5409.00

市场价格：￥4597.65

会员价格：￥4327.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Bone Sialoprotein II Antibody | AB1854 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Human and rat Bone sialoprotein (BSP). No cross reactivity with human osteopontin or osteonectin by RIA or ELISA. View All» Immunogen: Purified human Bone sialoprotein View All» Background Information: Bone sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning the bone turnover, pathological bone alterations as well as the rapid increase of osteoporosis make it necessary to establish new markers, Bone sialoprotein is discussed as a potential serum marker for monitoring bone remodelling. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Human and rat. Other species have not been tested View All» Application Notes: Western Blot Immunohistochemistry (Paraffin) (see suggested protocol below) Immunocytochemistry (1:100)RIA ELISA Optimal working dilutions must be determined by the end user. SUGGESTED STAINING PROCEDURE FOR RABBIT ANTI-BONE SIALOPROTEINAB1854 This protocol has been used successfully on 5 um, paraffin embedded sections from human fetal tibia, human fetal calvaria, human metastatic breast tumors to bone which were decalcified in 10% formic acid. 1. Dewaxing: Xylene 5 min. - Xylene 5 min. - Xylene 5 min. 2. Rehydration: 100% ethanol for 3 min. - 95% ethanol for 3 min. - 70% ethanol for 3 min. 3. Wash sections under running tap water for 5 minutes. 4. Wash sections in PBS. 5. Block endogenous peroxidase: 0.3% hydrogen peroxide in absolute methanol (2 mL of H2O2 30% in 198 mL methanol) for 30 minutes. (Cover the jar with foil, use stirring during incubation.) 6. Wash sections under running tap water for 10 minutes. 7. Mark tissue area with pap pen. 8. Cover the tissue area with 300 mL of blocking solution for 30 minutes at room temperature (blocking solution: 6 mL PBS, 0.3 gm BSA, 120 mL normal goat serum). 9. Shake off excess fluid (do not wash). 10. Incubate with primary antibody (AB1854 diluted 1:2,500 in PBS-T) at 2-8°C overnight in moist chamber. 11. Rinse with PBS-T, then wash 3 times in PBS-T each for 5 minutes at room temperature. 12. Incubate with biotinylated secondary antibody (for example Chemicon AP132B) for 30 minutes at room temperature. 13. Rinse with PBS-T, then wash in PBS-T 3 x 5 minutes at room temperature. 14. Incubate the sections with Vector ABC reagent for 30 minutes at room temperature. (ABC reagent: 2 drops of A and 2 drops of B, in 5 mL PBS, allow to stand 30 minutes at room temperature before use.) 15. Wash sections in PBS 3 x 5 minutes at room temperature. 16. Develop with DAB for 5-10 minutes. 17. Wash under running tap water. 18. Counter stain with Mayer's HX for 45 seconds, wash in warm tap water. 19. Dehydrate: 70% ethanol - 95% ethanol - 100% ethanol, 1 min. each. 20. Wash in Xylene, mount, dry. View All» Presentation: Lyophilized. Resuspend in 100 μL aqua bidest. Contains no preservatives. View All» Storage Conditions: Maintain at 2-8°C (lyophilized) or -20°C (reconstituted) for up to 6 months in undiluted aliquots. Avoid repeated freeze/thaw cycles. View All» UniProt Number: P21815 View All» Entrez Gene Number: NM_004967.2 View All» Gene Symbol:
  • IBSP

  • BNSP

  • BSP-II

  • BSP

  • SP-II
    View All» Alternate Names: BSPII; Integrin-binding Sialoprotein View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • ELISA

    • Immunocytochemistry

    • Immunohistochemistry

    • Immunohistochemistry (Paraffin)

    • Radioimmunoassay

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. View All» UniProt Summary: FUNCTION: SwissProt: P21815 # Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Promotes Arg-Gly-Asp-dependent cell attachment.SIZE: 317 amino acids; 35018 Da SUBCELLULAR LOCATION: Secreted.PTM: N-glycosylated; glycans consist of sialated and core- fucosylated bi-, tri- and tetraantennary chains. & O-glycosylated at eight sites; mucin-type glycans contain Gal, GlcNAc, GalNAc and terminal NeuAc.MISCELLANEOUS: It is possible that the segments of clustered carboxyl groups mediate the strong binding to hydroxyapatite. View All» Brand Family: Chemicon View All» Product Name: Anti-Bone Sialoprotein II Antibody | AB1854 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-152716.htm
      © Merck Millipore代理AB1854 Anti-Bone Sialoprotein II Antibody;store at -20℃，产品报价联系微信jinshanbio