货号：AB1783

品牌：Merck Millipore

规格：50UL

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Glutamate Transporter Antibody, Glial | AB1783 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Glial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining. View All» Molecular Weight: ~50 kDa View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide from the carboxy-terminus of rat GLT-1. View All» Background Information: Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Immunohistochemistry: 1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde. Immunohistochemistry: 1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody. Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended. Notes: Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. Optimal working dilutions must be determined by the end user. View All» Control: Rat brain tissue View All» Quality Assurance: Evaluated by Western Blot on mouse brain membrane lysates. View All» Purification Method: Unpurified View All» Presentation: Guinea pig antiserum containing no preservatives. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: P43004 View All» Entrez Gene Number: NM_004171.3 View All» Gene Symbol:
  • SLC1A2

  • GLT-1

  • EAAT2

  • GLT1
    View All» Alternate Names: GLT-1, EAAT2 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunohistochemistry (Paraffin)

    • Immunofluorescence

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. View All» UniProt Summary: FUNCTION: SwissProt: P43004 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.SIZE: 574 amino acids; 62104 Da SUBUNIT: Homotrimer.SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.PTM: Glycosylated.SIMILARITY: SwissProt: P43004 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. View All» Brand Family: Chemicon View All» Product Name: Anti-Glutamate Transporter Antibody, Glial | AB1783 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µL View All» Target / Localization: Plasma Membrane View All» Format: Serum View All» Host: Guinea Pig View All»
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