货号：AB175

品牌：Merck Millipore

规格：50UL

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GABA Antibody | AB175 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Chemicon (Millipore) View All» Specificity: Recognizes GABA. Staining was blocked by preabsorbing with 100 μM GABA conjugated to glutaraldehyde. 500 μM of similar conjugations of glutamic acid, glutamate and taurine failed to block staining. View All» Immunogen: GABA coupled to KLH via glutaraldehyde. View All» Species Reactivity: Mammals View All» Application Notes: Immunohistochemistry at 1:1,000 (FITC and peroxidase methods). Optimal working dilutions must be determined by the end user. Immunohistochemistry Protocal: Tissues fixed with 4% paraformaldehyde and 0-0.5% glutaraldehyde gives good results. Glutaraldehyde is required for antibody reactivity. 1) Tissue is fixed with 4% paraformaldehyde, 0-0.5% glutaraldehyde, 0.5% potassium dichromate in 0.1M phosphate buffer at pH 6.5. 2) Tissue is post-fixed overnight, vibratome sectioned in 50 mm and incubated in 0.05M Tris buffer, pH 6.5 for three hours. 3) Sections are incubated for 18-24 hours in AB175 diluted in PBS containing 0.1% sodium azide, 0.2% Triton X-100 and 1% normal goat serum. 4) Fluorescein conjugated antibody or ABC system may be used as the secondary reagent. Note: Without colchicine pretreatment well-stained cell bodies are visible in the cerebral cortex, cerebrallar cortex, superior colliculus and some brainstem raphe. With colchicine pretreatment, additional cell body staining is present in the interpeduncular nucleus and the dorsal column nuclei. View All» Control: Brain tissue View All» Purification Method: Unpurified View All» Presentation: Guinea Pig antiSerum. Liquid View All» Storage Conditions: Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Immunohistochemistry View All» Brand Family: Chemicon View All» Product Name: Anti-GABA Antibody | AB175 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 μL View All» Format: Serum View All» Host: Guinea Pig View All»