货号：46-710

品牌：Merck Millipore

规格：EA

目录价：￥3253.00

市场价格：￥2765.05

会员价格：￥2602.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: MILLIPLEX MAP Phospho Src (Tyr419) MAPmate, 46-710 View All» Trade Name: MILLIPLEX View All» Qty/Pk: 1 kit View All» Product Overview: The MILLIPLEX MAP Phospho Src (Tyr419) MAPmates pair is used in conjunction withthe MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602) to detectthe presence of phosphorylated Src (Tyr419) in cell lysates using the Luminex® 100™IS, 200™, or HTS system. Each MAPmates pair is ordered individually and may becombined for simultaneous multiplex analysis of cellular events. The MILLIPLEX MAPCell Signaling Buffer and Detection Kit is ordered separately and consists of a commonset of reagents needed for performing MAPmates assays. The detection assay is arapid, convenient alternative to Western Blotting and immunoprecipitation procedures.Each kit contains sufficient reagents for 100 individual assays. The MILLIPLEXMAP HeLa Cell Lysate: Unstimulated included in the MILLIPLEX MAP Cell Signaling Bufferand Detection Kit may be utilized as a negative control for this target, while the HeLaCell Lysate: Pervanadate included with this MAPmates pair may be utilized as a positivecontrol. View All» Background Information: Src:• Proto-oncogene• Nonreceptor Tyrosine Kinase• Src Family Kinase members include Src, Yes, Fyn, Fgr, Lck, Hck, Blk, and Lyn• Autophosphorylation of Tyr419 (activation site) and dephosphorylation of Tyr530 (inhibitory site) required to switch the kinase from the inactive closed formation to the active open formation• High levels of Src found in platelets, neural tissue and osteoclasts. • Mutations in this gene possibly involved in the malignant progression of colon cancer• Important for proliferation, adhesion, differentiation, migration and survival through activation of Ras/ERK, Akt, and STAT pathways View All» Key Applications: Multiplexing View All» Application Notes: • This assay requires 25 μL diluted cell lysate (recommended starting concentration is 20 μg cell lysate/well)• This is an overnight (4°C) or two-hour (RT) incubation assay; however, for best results an overnight incubation is recommended.• MAPmate is compatible with both Assay Buffer 1(Catalog # 43-010) and Assay Buffer 2 (Catalog # 43-041) View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Storage Conditions: Recommended storage for kit components is 2 - 8°C View All» Product Name: MILLIPLEX MAP Phospho Src (Tyr419) MAPmate, 46-710 View All» Materials Required but Not Delivered: • MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)• Cell lysates or cell extracts harboring protein(s) of interest prepared using the MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog # 48-602)• Protease inhibitors (Millipore Catalog #20-201 or equivalent)• Luminex Sheath Fluid (Luminex Catalog #40-50000) View All» Analytes Available: Src (Tyr419) View All» Configuration: Single Plex View All» Components:
  • MILLIPLEX MAP Anti-Src Beads (20X)

  • MILLIPLEX MAP Anti--phospho Src (Tyr419), biotin (20x)

  • MILLIPLEX MAP HeLa Cell Lysate: Pervanadate
    View All» UniProt Number: P12931 View All» Panel Type: Cell Signaling View All» Detection Methods: Luminex xMAP View All» Entrez Gene Number: NM_005417 View All»
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