货号：17-671

品牌：Merck Millipore

规格：10assays

目录价：￥6357.00

市场价格：￥5403.45

会员价格：￥5085.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Trimethyl-Histone H4 (Lys20) View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.The ChIPAb+ Trimethyl-Histone H4 (Lys20) set includes the Anti-trimethyl-Histone H4 (Lys20) antibody, a negative control supernatant, and qPCR primers which amplify a 110 bp region within the promoter of the humanβ-globin gene. The trimethyl histone H4 (Lys20) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of trimethyl-histone H4 (Lys20) associated chromatin. View All» Specificity: Recognizes histone H4, Mr 11 kDa View All» Molecular Weight: ~11 kDa View All» Epitope: a.a. 18-22 View All» Immunogen: The trimethyl histone H4 (Lys20) antibody is made against a synthetic peptide corresponding to amino acids 18-22 of histone H4. trimethylated on lysine 20. View All» Modifications: Methylation View All» Isotype: IgG View All» Species Reactivity:

• Human

• Green Plants

• Vertebrates
  View All» Species Reactivity Note: Human. Not tested in other species. The immunogen sequence is identical in a wide range of animal and plant species, so broad cross-reactivity is expected. View All» Application Notes: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (2 X 10⁶ cell equivalents per IP) was subjected to chromatin immunoprecipitation using 10 μL of either a negative control supernatant or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP A Kit (Part No. 17-610). Successful immunoprecipitation of trimethylhistone H4 (Lys20) associated DNA fragments was verified by qPCR using β-globin ChIP Primers versus Control Primers corresponding to the GAPDH promoter (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin, with immunoprecipitated DNA from negative control supernatant shown as (-) and trimethyl histone H4 (Lys20) shown as (+). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis:Acid extracts from HeLa cells untreated or pre-absorbed with 1 μM of trimethylated (Lys20) Histone H4 peptide (Lanes 1 and 2 respectively) were probed with anti-trimethyl-Histone H4 (Lys20) (1:1,000) (Please see figures). View All» Control: Includes negative control rabbit antibody and control primers specific for human β-globin promoter. View All» Quality Assurance: Chromatin Immunoprecipitation:Sonicated chromatin prepared from HeLa cells (1 X 10⁶ cell equivalents per IP) were subjected to chromatin immunoprecipitation using 10 µL of either a negative control supernatant, or Anti-Trimethyl-Histone H4 (Lys20) antibody and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of trimethyl-histone H4 (Lys20)-associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Presentation: Anti-trimethyl-Histone H4 (Lys20) (rabbit monoclonal IgG). One vial containing 100 μL of cell culture supernatant in 0.1% sodium azide. Store at -20°C. Negative Control Supernatant. One vial containing 100 μL cell rabbit monoclonal culture supernatant containing 0.1% sodium azide. Store at -20°C.ChIP Primers B-Globin. One vial containing 75 μL of 5 μM of each primer specific for human β-globin. Store at -20°C.FOR: AGG ACA GGT ACG GCT GTC ATCREV: TTT ATG CCC AGC CCT GGC TC View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P62805 View All» Entrez Gene Number: NM_003538.3 View All» Gene Symbol:
  • H4/A

  • H4/B

  • H4/C

  • H4/D

  • H4/E

  • H4/G

  • H4/H

  • H4/I

  • H4/J

  • H4/J

  • H4/M

  • H4/N

  • H4/O

  • H4/a

  • H4F2

  • H4FA

  • H4FB

  • H4FC

  • H4FD

  • H4FE

  • H4FG

  • H4FH

  • H4FI

  • H4FJ

  • H4FK

  • H4FM

  • H4FN

  • H4FO

  • HIST2H4
    View All» Alternate Names:
    • H4K20me3

    • Histone H4 (tri methyl K20)

    • H4 histone family, member A

    • histone 1, H4a

    • histone cluster 1, H4a
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Dot Blot
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus. PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.Citrullination at Arg-4 by PADI4 impairs methylation.Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing. Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Sumoylated, which is associated with transcriptional repression. SEQUENCE SIMILARITY: Belongs to the histone H4 family.SEQUENCE CAUTION:The sequence AAI28106.1 differs from that shown. Reason: Frameshift at position 3. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ Trimethyl-Histone H4 (Lys20) View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 10 assays View All» Host: Rabbit View All» Packaging: 10 assays per set. Recommended use: ~10 μL antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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