货号：17-615

品牌：Merck Millipore

规格：125Ug

目录价：￥6227.00

市场价格：￥5292.95

会员价格：￥4981.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Acetyl-Histone H3 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Acetyl-Histone H3 set contains purified rabbit polyclonal antibody and the normal rabbit IgG antibody, which can be used to demonstrate that the acetyl-histone H3 antibody is capable of precipitating acetyl-histone H3 associated chromatin. The qPCR primers included flank the human GAPDH promoter and produce a 166 base pair PCR product. View All» Specificity: Recognizes acetyl-histone H3 View All» Molecular Weight: 17 kDa View All» Immunogen: The acetyl-histone H3 antibody is made against a peptide corresponding to amino acids 1-20 of Tetrahymena histone H3 ARTKQTAR[K*]STGG[K*]APRKQL(C) where K* is acetylated) View All» Species Reactivity:

• Human

• Mouse

• Tetrahymena
  View All» Species Reactivity Note: Proven to react with human and mouse. Tetrahymena predicted to cross-react based upon sequence of antibody generating peptide. View All» Application Notes: Western Blot Analysis:Acid-extracted proteins from HeLa cells treated with 5 mM sodium butyrate for 24 hours (Lane 2) and untreated HeLa cells (Lane 1) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-acetyl-Histone H3 (0.01 μg/mL).Proteins were visualized using a goat-rabbit secondary antibody conjugated to HRP and a chemilumnescence detection system (Please see figures). View All» Control: Included negative control antibody rabbit IgG and control primers specific for human GAPDH promoter. View All» Quality Assurance: Routinely evaluated by chromatin immunoprecipitation on HeLa nuclear extract. View All» Purification Method: Protein A purfied View All» Presentation: Anti-Acetyl-Histone H3 (rabbit polyclonal IgG). One vial containing 125 ug of protein A purified antibody in 125 μL of storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Store at -20°C.Normal Rabbit IgG. One vial containing 125 ug of normal rabbit IgG in 125 uL volume. Store at -20°C.Control Primers. One vial containing 75 μL of 5 μM of each primer specific for for human GAPDH. Store at -20°C.FOR: TAC TAG CGG TTT TAC GGG CGREV: TCG AAC AGG AGG AGC AGA GAGCGA View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. View All» UniProt Number: P68431 View All» Entrez Gene Number: NP_003484 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunocytochemistry
    View All» Brand Family: Upstate View All» Product Name: ChIPAb+ Acetyl-Histone H3 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 125 μg View All» Format: Purified View All» Host: Rabbit View All» Packaging: 25 assays per set. ~5 μg per chromatin immunoprecipitation View All»
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    © Merck Millipore代理17-615 ChIPAb+ Acetyl-Histone H3 - ChIP Validated Antibody and Primer Set;ChIPAb+ 乙酰化组蛋白H3 - ChIP经验证的抗体和引物组，产品报价联系微信jinshanbio