货号：17-601

品牌：Merck Millipore

规格：25assays

目录价：￥6357.00

市场价格：￥5403.45

会员价格：￥5085.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+ Sp1 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. Sp1 antibody and the negative control antibody (rabbit normal IgG) can be used to demonstrate that the Sp1 antibody is functionally validated in the precipitation of Sp1 associated chromatin. The qPCR primers included flank the Sp1 binding site in human DHFR promoter. View All» Specificity: Sp1 View All» Molecular Weight: 105 kDa and 95 kDa View All» Immunogen: Full length human Sp1 protein View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Application Notes: Western Blot Analysis:HeLa nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Sp1 (0.2 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures). View All» Control: Includes negative control rabbit IgG and control primers specific for human dihydrofolate reductase (DHFR) promoter. View All» Quality Assurance: Chromatin immunoprecipitation:Sonicated chromatin prepared from 1 x 10⁶ HeLa cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Rabbit Normal IgG or Rabbit Anti-Sp1 and the Magna ChIPTM A kit (Cat.# 17-610) Rapid Protocol (2 hour IP). Successful enrichment of Sp1 associated DNA fragments was verified by qPCR using ChIP Primers DHFR flanking the human DHFR promoter that contains a Sp1 binding site (Please see figures).Please refer to the EZ-Magna ChIP™ A (Cat.# 17-408)or EZ-ChIP™ (Cat.# 17-371) kit protocols forexperimental details. View All» Purification Method: Protein A purfied View All» Presentation: Anti-Sp1 (Rabbit polyclonal IgG). 1 vial containing 100 μg of protein A purified IgG in 100 μL of 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Store at -20°C. The Sp1 antibody is made against full length human Sp1 protein and can recognize Sp1, MW 105 kDa and 95 kDa, of human, mouse and rat origins.Normal Rabbit IgG. One vial containing 125 μg of normal rabbit IgG in 125 μL. Store at -20°C.ChIP primers DHFR. 1 vial containing 75 μL of 5 μM of each control primer specific for human dihydrofolate reductase (DHFR) promoter. Store at -20°C.FOR: TCG CCT GCA CAA ATA GGG ACREV: AGA ACG CGC GGT CAA GTT T View All» Storage Conditions: 1 year at -20°C from date of shipment. View All» UniProt Number: P08047 View All» Entrez Gene Number: NM_138473.2 View All» Gene Symbol:
  • SP1

  • TSFP1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: SwissProt: P08047 # Binds to GC box promoters elements and selectively activates mRNA synthesis from genes that contain functional recognition sites. Can interact with G/C-rich motifs from serotonin receptor promoter.SIZE: 785 amino acids; 80693 Da SUBUNIT: Interacts with ATF7IP, ATF7IP2, POGZ, HCFC1, AATF and PHC2. Interacts with varicella-zoster virus IE62 protein and HIV-1 Vpr.SUBCELLULAR LOCATION: Nucleus.PTM: O-glycosylated; contains N-acetylglucosamine side chains.SIMILARITY: SwissProt: P08047 ## Belongs to the Sp1 C2H2-type zinc-finger protein family. & Contains 3 C2H2-type zinc fingers. View All» Brand Family: Upstate View All» Product Name: ChIPAb+ Sp1 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Purified View All» Host: Rabbit View All» Packaging: 25 assays per kit, ~4μg per chromatin immunoprecipitation. View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://16ao.com/thread-147976.htm
    © Merck Millipore代理17-601 ChIPAb+ Sp1 - ChIP Validated Antibody and Primer Set;ChIPAb+ Sp1 - ChIP经验证的抗体和引物组store at -20℃，产品报价联系微信jinshanbio