货号：17-488

品牌：Merck Millipore

规格：EA

目录价：￥6458.00

市场价格：￥5489.30

会员价格：￥5166.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: phospho-p38α (Thr180/Tyr182) STAR ELISA Kit View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 96 assays View All» Background Information: I. TEST PRINCIPLE The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The p38 MAPK plate is coated with a specific mouse monoclonal p38 MAPK capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incubated in the microwells allowing p38 MAPK antigen to be captured in the plate wells. The plate is then washed to remove any non-bound unspecific material. The wells are then incubated with a specific rabbit anti-phospho-p38 (Thr180/Tyr182) antibody to detect the captured p38 (Thr180/Tyr182) on the plate well. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard line of detection. II. p38 BACKGROUND The p38 MAPK family of Ser/Thr kinase proteins is made up of 4 isoforms, p38alpha (MAPK14 and SAPK2a), p38beta (MAPK11 and SAPK2b), p38delta (MAPK13, SAPK4), and p38gamma (MAPK12, SAPK3). The other p38 isoforms share 74%, 57%, and 60% sequence identity with the alpha isoform, respectively. All four isoforms posses the TGY motif and are activated by the dual phosphorylation of the threonine and tyrosine residues in this motif, which correspond to Thr180/Tyr182 in human p38alpha. p38, especially the alpha and beta isoforms, are able to phosphorylate a wide array of targets including numerous kinases including MAPKAP-K2, MAPKAP-K3, PRAK, MSK, and MNK as well as transcription factors including STAT1, p53, NFATp, ATF-2, Elk-1 (although weakly), and MEF2a/c. p38 MAPK family members have traditionally been associated with stress, immune response, and regulation of cell proliferation and apoptosis. They also have a role in regulating the production of pro-inflammatory cytokines and have been associated with diseases such as asthma and several autoimmune diseases, including rheumatoid arthritis, diabetes, and inflammatory bowel disease. Just as ERK is phosphorylated and activated by MEK, p38alpha MAP Kinase is activated predominantly by its upstream regulatory kinases MKK6 and MKK3 as well as MKK4 which also activates JNK, but is not a substrate for MKK7 like JNK is. View All» Key Applications: ELISA View All» Species Reactivity: Human View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. View All» UniProt Summary: FUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.COFACTOR: Magnesium.SIZE: 360 amino acids; 41293 Da SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Sensitivity: Sensitivity: 1 Unit/mL. Range of Detection: 1.6 to 100 Units/mL Species Reactivity: Human View All» Storage Conditions: Maintain the unopened kit at 2-8°C until expiration date. Precautions • The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data. • Caustic Material: Stop Solution. Caution: Eye, hand, face, and clothing protection should be worn when handling this material. • Safety Warnings and Precautions: This kit is designed for research use only and not recommended for internal use in humans or animals. All chemicals should be considered potentially hazardous and principles of good laboratory practice should be followed. • The Detection Antibody and ELISA Diluent contain sodium azide. Sodium azide may react with copper and lead plumbing to form highly explosive metal azides. Upon disposal, flush with large amounts of water to prevent azide build-up. Avoid contact with skin. • The Anti-Rabbit IgG HRP Conjugate and HRP Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly. View All» Modifications: Phosphorylation View All» Gene Symbol:

• MAPK14

• PRKM14

• RK

• SAPK2A

• CSBP1

• p38

• CSPB1

• CSBP

• PRKM15

• Mxi2

• p38ALPHA

• MXI2

• EXIP

• CSBP2
  View All» Product Name: phospho-p38α (Thr180/Tyr182) STAR ELISA Kit View All» Materials Required but Not Delivered: 1. Multi-channel or repeating pipettes 2. Plate shaker (optional) 3. Pipettors & tips capable of accurately measuring 1-1000 µL 4. Graduated serological pipettes 5. 96-well microtiter Plate Reader with 450 nm filter 6. Graphing software for plotting data or graph paper for manual plotting of data 7. Microfuge tubes for standard and sample dilutions 8. Mechanical vortex 9. 1 liter container 10. Distilled or deionized water View All» Analytes Available: p38/SAPK View All» Components:
  • Capture Plate pre-coated with anti-p38alpha MAPK antibody: (Part No. 17-487A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.

  • Anti-phospho-p38alpha (Thr180/Tyr182) detection antibody: (Part No. 17-487B) One bottle (11 mL) of anti-phospho-p38alpha (Thr180/Tyr182) detection antibody containing sodium azide, ready to use.

  • ELISA Diluent: (Part No. 17-487C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.

  • 25X ELISA Wash Buffer: (Part No. 17-487D) One bottle (50 mL) of 25X ELISA Wash Buffer.

  • Anti-Rabbit IgG HRP conjugate: (Part No. 17-487E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosal.

  • HRP Diluent: (Part No. 17-487F) One bottle (25 mL) of HRP Diluent containing thimerosal.

  • TMB Solution: (Part No. 17-487G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.

  • Stop Solution: (Part No. 17-487H) One bottle (25 mL) of stop solution, ready to use.

  • phospho-p38alpha (Thr180/Tyr182) Standard: (Part No. 17-487I) Two vials of phospho-p38alpha (Thr180/Tyr182) standard, lyophilized.

  • Plate Covers: Two plate covers.
    View All» UniProt Number: Q16539 View All» Detection Methods: Colorimetric View All» Entrez Gene Number:
    • NM_001315.1

    • NM_139012.1

    • NM_139013.1

    • NM_139014.1
      View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://16ao.com/thread-147926.htm
      © Merck Millipore代理17-488 STAR phospho-p38a (Thr180/Tyr182) ELISA，产品报价联系微信jinshanbio