货号：17-484

品牌：Merck Millipore

规格：EA

目录价：￥6330.00

市场价格：￥5380.50

会员价格：￥5064.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: phospho-IR (β Subunit) (Tyr 1162/1163) STAR ELISA Assay Kit View All» Trade Name:

• STAR ELISA

• Upstate (Millipore)
  View All» Qty/Pk: 96 assays View All» Background Information: I. TEST PRINCIPLE The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IR plate is coated with a specific mouse monoclonal IR (β subunit) capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standards included in the kit are incubated in the microwells allowing IR to be captured in the plate wells. The plate is then washed to remove any unbound non-specific material. The wells are then incubated with a specific rabbit anti-phospho-IR antibody to detect the captured IR phosphorylated on Tyrosines 1162 and 1163. The unbound detection antibody is washed away followed by incubation with an HRP-conjugated anti-rabbit antibody. This allows for a sensitive enzymatic detection of the sample. After the addition of TMB substrate and stop solution the absorbance is measured at 450 nm using a plate reader. The entire assay takes less than 5 hours to complete with minimal hands-on time. Many of the reagents are supplied in ready-to use formulations for ease of use. The kit also includes a standard that is run as both a positive control and to develop a standard curve. II. IR BACKGROUND The Insulin Receptor (IR) is synthesized as a single polypeptide, which is subsequently cleaved to generate an extracellular α chain and a transmembrane and intracellular β chain, tethered together by disulfide bonds. The β chain has multiple tyrosine phosphorylation sites, including three autophosphorylation sites at its activation loop. Insulin stimulates the signaling cascade by binding to the insulin receptor through its extracellular α subunits. This stimulates the tyrosine kinase activity of the β subunits of the receptor. The receptor then autophosphorylates itself and phosphorylates the IRS (Insulin Receptor Substrate) proteins that are very important modulators of insulin signaling. Phosphorylated IRS, in turn, interacts with several downstream effectors of insulin signaling, including PI3 Kinase p85, GRB2, SHP2, Nck, Crk, and Fyn. The overall structure of the IR is highly homologous to the IGF-I Receptor, except in their c-termini, where the two proteins diverge somewhat. Insulin signaling is highly dependent on the PI3 Kinase pathway and Akt, which appear to mediate the functions of insulin. View All» Key Applications: ELISA View All» Species Reactivity:
  • Human

  • Mouse

  • Rat
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: After removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: P06213 # This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.SIZE: 1382 amino acids; 156307 Da SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7.SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.TISSUE SPECIFICITY: Isoform Long and isoform Short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin. Isoform Short is expressed also in the spleen and lymphoblasts.PTM: After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. & Autophosphorylated on tyrosine residues in response to insulin. & Phosphorylation of Tyr-999 is required for IRS1- and SHC1- binding.DISEASE: SwissProt: P06213 # Defects in INSR are the cause of insulin resistance (Ins resistance) [MIM:125853]. & Defects in INSR are the cause of Rabson-Mendenhall syndrome [MIM:262190]; also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin- resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. & Defects in INSR are the cause of leprechaunism [MIM:246200]; also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. & Defects in INSR may be associated with noninsulin- dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. & Defects in INSR are the cause of familial hyperinsulinemic hypoglycemia 5 (HHF5) [MIM:609968]. Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. & Defects in INSR are the cause of insulin-resistant diabetes mellitus with acanthosis nigricans type A (IRAN type A) [MIM:610549]. This syndrome is characterized by the association of severe insulin resistance (manifested by marked hyperinsulinemia and a failure to respond to exogenous insulin) with the skin lesion acanthosis nigricans and ovarian hyperandrogenism in adolescent female subjects. Women frequently present with hirsutism, acne, amenorrhea or oligomenorrhea, and virilization. This syndrome is different from the type B that has been demonstrated to be secondary to the presence of circulating autoantibodies against the insulin receptor.SIMILARITY: SwissProt: P06213 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Sensitivity: Sensitivity: 1 Unit/mL Range of Detection: 1.6 - 100 Units/mL View All» Storage Conditions: Maintain the unopened kit at 2-8°C until expiration date. View All» Modifications: Phosphorylation View All» Gene Symbol:
    • INSR

    • CD220

    • IR

    • HHF5
      View All» Product Name: phospho-Insulin Receptor, β subunit STAR ELISA Assay Kit View All» Alternate Names:
      • Insulin Receptor

      • IR
        View All» Materials Required but Not Delivered: 1. Multi-channel or repeating pipettes 2. Plate shaker (optional) 3. Pipettors & tips capable of accurately measuring 1-1000 µL 4. Graduated serological pipettes 5. 96-well microtiter Plate Reader with 450 nm filter 6. Graphing software for plotting data or graph paper for manual plotting of data 7. Microfuge tubes for standard and sample dilutions 8. Mechanical vortex 9. 1 liter container 10. Distilled or deionized water View All» Analytes Available: Insulin View All» Quality Assurance: Routinely evaluated by ELISA. View All» Components:
        • Capture Plate pre-coated with anti-IR antibody: (Part No. 17-483A) One pre-coated 96-stripwell immunoplate sealed in a foil pouch.

        • Anti-phospho-IR (Tyr 1162/1163) detection antibody: (Part No. 17-483B) One bottle (11 mL) of anti-phospho-IR (Tyr 1162/1163) detection antibody containing sodium azide, ready to use.

        • ELISA Diluent: (Part No. 17-483C) One bottle (25 mL) of ELISA Diluent containing sodium azide, ready to use.

        • 25X ELISA Wash Buffer: (Part No. 17-483D) One bottle (50 mL) of 25X ELISA Wash Buffer.

        • Anti-Rabbit IgG HRP conjugate: (Part No. 17-483E) One vial (125 µL) of 100X anti-rabbit HRP conjugate containing thimerosal.

        • HRP Diluent: (Part No. 17-483F) One bottle (25 mL) of HRP Diluent containing thimerosal.

        • TMB Solution: (Part No. 17-483G) One bottle (25 mL) of stabilized tetramethylbenzidine (TMB), ready to use.

        • Stop Solution: (Part No. 17-483H) One bottle (25 mL) of stop solution, ready to use.

        • phospho-IR Standard: (Part No. 17-483I) Two vials of IR ((Tyr 1162/1163) standard, lyophilized.

        • Plate Covers: Two plate covers.
          View All» UniProt Number: P06213 View All» Detection Methods: Chromogenic View All» Entrez Gene Number:
          • NM_000208.2

          • NM_001079817.1
            View All»
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