货号：17-315

品牌：Merck Millipore

规格：EA

目录价：￥8060.00

市场价格：￥6851.00

会员价格：￥6448.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Tyrosine Kinase Assay Kit, Colorimetric Detection View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 1 kit View All» Product Overview: This non-radioactive kinase assay kit is a two-stage peptide phosphorylation/colorimetric detection assay kit. In the first stage, a biotinylated substrate peptide containing tandem repeats of Poly (Glu₄-Tyr) is incubated with a tyrosine kinase enzyme sample in the presence of non-radioactive ATP and a Mn²⁺/Mg²⁺ co-factor cocktail. The kinase assay can be performed with substrate peptide either in solution, or bound to the well of a streptavidin-coated 96-well plate. The second stage involves the detection of phosphorylated substrate by Direct Enzyme Linked Immunosorbent Assay (ELISA) using a monoclonal anti-phosphotyrosine-HRP (Horse Radish Peroxidase) antibody conjugate. Tetramethylbenzidine (TMB) is provided as the HRP substrate. View All» Key Applications: Enzyme Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Quality Assurance: Routinely evaluated by a reference peptide titration. Increasing amounts of Poly (Glu₄-phospho-Tyr) Peptide, biotinylated were bound to streptavidin-coated wells. Total peptide mass/well was adjusted by the addition of Poly (Glu₄-Tyr) Peptide, biotinylated to a well. Bound phospho-tyrosine containing peptide was detected with Anti-Phosphotyrosine, recombinant 4G10, HRP conjugate. View All» Components:

• Streptavidin Coated Microwell Plate (Cat.# 20-183)

• Anti-pTyr, recombinant 4G10™, HRP

• ELISA wash and blocking buffers

• Poly (Glu4-Phospho-Tyr), biotinylated

• Poly (Glu4-Tyr), biotinylated

• Tyrosine Kinase Reaction Buffer, 5X

• Sodium Orthovanadate, 50mM
  View All» Brand Family: Upstate View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Detection Methods:
  • Colorimetric

  • Colorimetric
    View All» Product Name: Tyrosine Kinase Assay Kit, Colorimetric Detection View All» Analytes Available: Tyrosine Kinase View All»
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