货号：17-10252

品牌：Merck Millipore

规格：

目录价：￥4960.00

市场价格：￥4216.00

会员价格：￥3968.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+™ LSF View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ LSF set includes the LSF antibody, a Normal rabbit IgG, and control primers which amplify a 148 bp region of ChIP Primers, Mouse switch (S) regions α. The LSF and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of LSF-associated chromatin. View All» Molecular Weight: ~57 kDa observed. Four isoforms are known to exist, isoforms 1 and 4 (~57 kDa) and isoforms 2 and 3 (~51 kDa). View All» Immunogen: Linear peptide corresponding to amino acids 310-335 of human LSF. View All» Background Information: Transcription factor LSF (late simian virus 40 factor) also known as transcription factor LSF, CP2, LBP-1c, and SEF is expressed in a variety of cell types including brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung. LSF is essential for cell cycle progression at the G1/S transition after reentry into the cell cycle from G0. LSF plays an important role in DNA synthesis and cell survival. A major cellular target of LSF is the thymidylate synthase (TS) gene coding for the rate limiting enzyme in the production of dTTP. Disruption of LSF function results in apoptosis at S phase or to cell cycle arrest at the G1/S transition. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with Mouse. Predicted to react with Human based on immunogen design. View All» Application Notes: Chromatin Immunoprecipitation: Representative lot data.Sonicated chromatin prepared from mouse brain tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal rabbit IgG, or 2 µL of Anti-LSF and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of LSF associated DNA fragments was verified by qPCR using ChIP Primers, Mouse switch (S) regions α as a positive locus, and mouse GAPDH promoter as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.Western Blot Analysis: Representative lot data.Mouse brain tissue lysate was probed with Anti-LSF (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LSF (~57 kDa). (Figure 3). View All» Control: Includes normal rabbit IgG and primers specific for Mouse switch (S) regions α. View All» Quality Assurance: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from mouse brain tissue (1 mg tissue equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal rabbit IgG, or 2 µL of Anti-LSF and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of LSF associated DNA fragments was verified by qPCR using ChIP Primers, Mouse switch (S) regions α (Figure 1). Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. View All» Purification Method: Affinity purified View All» Presentation: Anti-LSF (rabbit polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before the addition of glycerol to 30%. Store at -20° C.Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.ChIP Primers, Mouse switch (S) regions α. One vial containing 75 μL of 5 μM of each primer specific for Mouse switch (S) regions α. Store at -20°C.FOR: CTA GAC CGG GCA GAC CTA GAREV: AAA GCC CAT TCC AGC CTA GT View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q12800 View All» Entrez Gene Number: NP_005644 View All» Gene Symbol:
  • TFCP2

  • LSF

  • SEF
    View All» Alternate Names:
    • Alpha-globin transcription factor CP2

    • SAA3 enhancer factor

    • Transcription factor LSF
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Summary: This gene encodes a transcription factor that binds the alpha-globin promoter and activates transcription of the alpha-globin gene. The encoded protein regulates erythroid gene expression, plays a role in the transcriptional switch of globin gene promoters, and it activates many other cellular and viral gene promoters. The gene product interacts with certain inflammatory response factors, and polymorphisms of this gene may be involved in the pathogenesis of Alzheimer's disease. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Binds a variety of cellular and viral promoters including fibrinogen, alpha-globin, SV40 and HIV-1 promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 By similarity. Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter.SUBUNIT STRUCTURE: Binds to DNA as a dimer, isoform 3 does not bind to DNA or affect the binding of isoform 1 to DNA. Interacts with UBP1 and PIAS1, and is probably part of a complex containing TFCP2, UBP1 and PIAS1 (by similarity). Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4.SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Ubiquitous. Expressed in brain, ovary, kidney, thymus, spleen, liver, adrenal, heart and lung (at protein level).DEVELOPMENTAL STAGE: Expressed in fetal erythroid tissue.MISCELLANEOUS: Authors noted that a 10-fold molar excess of isoform 3 over isoform 1 inhibited DNA-binding.SEQUENCE SIMILARITIES: Belongs to the grh/CP2 family. CP2 subfamily. View All» Product Name: ChIPAb+™ LSF View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Affinity Purified View All» Host: Rabbit View All» Packaging: 25 assays per set. Recommended use: ~2 μL of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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