货号：17-10246

品牌：Merck Millipore

规格：

目录价：￥4680.00

市场价格：￥3978.00

会员价格：￥3744.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: ChIPAb+™ Histone H2A.Bbd View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Product Overview: All ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction. The ChIPAb+ Histone H2A.Bbd set includes the Histone H2A.Bbd antibody, a Normal Rabbit IgG, and control primers which amplify a 70 bp region of ChIP primers, PGK1. The Histone H2A.Bbd and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of Histone H2A.Bbd -associated chromatin. View All» Specificity: This antibody recognizes human Histone H2A.Bbd. View All» Molecular Weight: ~17 kDa observed View All» Epitope: a.a. 35-55 View All» Immunogen: KLH-conjugated linear peptide corresponding to human Histone H2A.Bbd. View All» Background Information: Histone H2A.Bbd (Barr body-deficient), also known as histone H2A-Bbd type 1, is an atypical histone variant. This histone variant associates with H2B and replaces histone H2A in the nucleosome. In contrast to other H2A histones, this variant lacks conserved residues that are targets of post-translational modifications. Consequently this H2A.Bbd variant is about 15 amino acids shorter and only 48% similar to the canonical H2A. The major differences between the two are found in the docking domain that links H2A -H2B to the H3-H4 tetramer. Nucleosomes containing this histone adopt a more relaxed structure and protect ~118 base pairs of DNA instead of the 147 base pairs found in classical nucleosomes. This variant histone is associated with active X chromosomes and autosomes, and is typically absent from inactive X chromosomes. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. View All» Application Notes: Western Blot Analysis: Representative lot data.H2A.Bbd overexpression (Lane 1-2), various recombinant Histone proteins (Lane 4-7) and HeLa acid extract (Lane 8) lysates were probed with Anti-Histone H2A.Bbd (0.5 µg/mL); Lane 1: 500 ng of H2A.Bbd overexpression lysate Lane 2: 1 µg H2A.Bbd overexpression lysate Lane 3: 500 ng Vector transfected cell lysate control Lane 4: 500 ng Recombinant Histone H2A (Cat.#: 14-493) Lane 5: 500 ng Recombinant Histone H2B (Cat.#: 14-491) Lane 6: 500 ng Recombinant Histone H3 (Cat.#: 14-494) Lane 7: 500 ng Recombinant Histone H4 (Cat.#: 14-697)Lane 8: 10 µg HeLa acid extract Proteins were visualized using a Donkey Anti Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates Histone H2A.Bbd (~17 kDa). (Figure 2). View All» Control: Includes normal rabbit IgG and primers specific for human PGK1. View All» Quality Assurance: Chromatin Immunoprecipitation:Representative lot data.Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 4 µg of either Normal Rabbit IgG, or 4 µg of Anti-Histone H2A.Bbd, and the buffers from the EZ-Magna ChIP™ A Kit (Cat. # 17-408) plus the 16-663 Magna ChIP Protein A+G Beads. Successful immunoprecipitation of Histone H2A.Bbd associated DNA fragments was verified by qPCR using ChIP primers specific for the human PGK1 region as a positive locus (Figure 1). View All» Purification Method: Affinity purified View All» Presentation: Anti-Histone H2A.Bbd (rabbit polyclonal). One vial containing 100 µg of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Store at -20°CConcentration: 0.7 mg/mL Normal Rabbit IgG, . One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.ChIP primers, PGK1. One vial containing 75 μL of 5 μM of each primer specific for human PGK1. Store at -20°C.FOR: CCT TCC CCC ACG CTC CTAREV: TTG TGC AGG AAC CTG TGA GAT G View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P0C5Y9 View All» Entrez Gene Number: NP_001017990 View All» Gene Symbol:
  • H2A.Bbd

  • H2AFB1
    View All» Alternate Names:
    • H2A histone family, member B1

    • H2A Barr body-deficient

    • histone H2A-Bbd type 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. This gene is part of a region that is repeated three times on chromosome X, once in intron 22 of the F8 gene and twice closer to the Xq telomere. This record represents the most centromeric copy which is in intron 22 of the F8 gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Atypical histone H2A which can replace conventional H2A in some nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Nucleosomes containing this histone are less rigid and organize only 118 base pair of DNA instead of 147 in classical nucleosomes. They are associated with transcriptionally active chromatin and excluded form Barr bodies. Ref.4 Ref.5SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. May be incorporated into a proportion of nucleosomes, replacing one or more H2A molecules.SUBCELLULAR LOCATION: Nucleus. Chromosome. Note: Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies.DOMAIN: The docking domain is responsible for the weaker heterodimerization with H2B. Ref.4MISCELLANEOUS: In contrast to other H2A histones, it does not contain the conserved residues that are the target of post-translational modifications.SEQUENCE SIMILARITIES: Belongs to the histone H2A family. View All» Product Name: ChIPAb+™ Histone H2A.Bbd View All» Concentration: 0.7 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 25 assays View All» Format: Affinity Purified View All» Host: Rabbit View All» Packaging: 25 assays per set. Recommended use: ~4 μg of antibody per chromatin immunoprecipitation (dependent upon biological context). View All»
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