货号：16-668

品牌：Merck Millipore

规格：

目录价：￥3552.00

市场价格：￥3019.20

会员价格：￥2841.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: AbSurance™ Complete Core Histone Antibody Specificity Array View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 2 Arrays View All» Product Overview: Features and Benefits

• Simple Western blot-like detection procedure

• High quality ( 〉95 % purity) peptides analyzed by mass spectrometry

• A total of 89 peptides on two membranes, for easy screening of histone H3, or H2A, H2B, and H4 peptides

• Two concentrations (10 ng and 100 ng) of each peptide allows detection of both strong and weak cross-reactivity

• Consistent and uniform spotting of peptides using proprietary process

• Data collection using either film or standard CCD imaging systems

• Easy- to-read 8 x 12 macroarray – no special imaging equipment or software required

• PVDF-FL membrane allows sensitive chemiluminescent or fluorescent detection

• Positive control IgG spots and membrane landmarks for simple and easy spot identification
  The AbSurance™ Complete Core Histone Antibody Specificity Array is comprised of 89 high quality modified and unmodified histone peptides ( 〉95 % purity) spotted on Immobilon®-FL PVDF membranes in a 96-well format. One membrane contains 46 histone H3 peptides (8 acetylated, 27 methylated, 5 phosphorylated, and 6 unmodified). The other membrane contains 43 histone H2A, H2B, H4 peptides (13 acetylated, 16 methylated, 6 phosphorylated, and 8 unmodified). Both membranes in the AbSurance™ Complete Core Histone Antibody Specificity Array kit contain 100 ng and 10 ng quantities of each peptide, in addition to 10 ng each of positive control IgG from rat, mouse, sheep, and rabbit. The control IgG, and a cut corner at the A1 position of each membrane, can be used as location reference marks for easy identification of spots. Peptides are evenly distributed on the array to enable quantification of distinct spots, and accurate determination of cross-reactivity. The format of the AbSurance™ Complete Core Histone Antibody Specificity Array enables a Western-blot–like detection approach using standard chemiluminescent reagents followed by image capture with either film or a CCD imaging system. View All» Applications: Histone Antibody Specificity Screening, Chromatin Immunoprecipitation View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial View All» Presentation: Immobilon®-FL PVDF membranes View All» Storage Conditions: Store at room temperature up to 6 months View All» Detection Methods:
  • Chemiluminescence

  • Fluorescence
    View All» Modifications:
    • Acetylation

    • Methylation

    • Phosphorylation
      View All» Product Name: AbSurance™ Complete Core Histone Antibody Specificity Array View All» Materials Required but Not Delivered:
      • Primary Antibody

      • Secondary Antibody

      • Methanol

      • TBST Solution (Tris-HCl (ph 7.4); 0.15 M NaCl, and 0.05% Tween® 20)

      • Blocking Solution (5 % non-fat dried milk in TBST)

      • Immobilon® Western Chemiluminescent HRP Substrate (Cat. No. WBKLS0100) or RapidStep™ ECL Reagent (Cat. No. 345818-100ML)

      • Containers for the membrane (e.g. Nunc® dishes, Cat. No. 267060)

      • Shaker platform

      • Micropipettes and pipette tips

      • Film or Imager (e.g. Bio-Rad ChemiDoc™ System)
        View All»
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