货号：16-103

品牌：Merck Millipore

规格：100Ug

目录价：￥8738.00

市场价格：￥7427.30

会员价格：￥6990.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate | 16-103 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: Anti-phosphotyrosine monoclonal antibody, clone 4G10 (cat.# 05-321), cross-linked to biotin. View All» Specificity: Recognizes tyrosine-phosphorylated proteins from all species. View All» Molecular Weight: Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. View All» Epitope: N/A View All» Immunogen: Phosphotyramine-KLH. View All» Modifications: Phosphorylation View All» Clone: 4G10® View All» Isotype: IgG2bκ View All» Background Information: Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in response to various treatments.DescriptionAnti-phosphotyrosine monoclonal antibody, clone 4G10® (cat. # 05-321), cross-linked to biotin. View All» Species Reactivity: All View All» Species Reactivity Note: Human. Expected to cross-react with rat and mouse. View All» Application Notes: Immunoprecipitation: 4 μg of a previous lot, used in conjunction with Streptavidin, agarose conjugate (Catalog # 16-126), immunoprecipitated phosphotyrosine containing proteins from a lysate of EGFstimulated A431 cells. Note: To preserve phosphotyrosine, add 0.2 mM sodium orthovanadate to the lysis buffer.Immunocytochemistry: 5-10 μg/mL of a previous lot gave positive immunostaining of EGF-stimulated A431 cells that had been fixed with ethanol:acetic acid [1:1].Application NotesFor use in applications in which a biotin conjugate is advantageous, such as WB and IC. View All» Control: Pervanadate-treated human A431 cell extracts or EGF-treated human A431 cells View All» Quality Assurance: Routinely evaluated by Western Blot on EGF treated A431 lysates.Western Blot Analysis: 1:500 dilution of this lot detected Tyrosine phosphorylated proteins on 10 μg of EGF treated A431 lysates. View All» Purification Method: Protein G purfied View All» Presentation: PBS containing 0.05% sodium azide. View All» Storage Conditions: 9 months at 2-8°C from date of shipment. NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial prior to removing the cap. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Immunoprecipitation

• Western Blotting
  View All» Brand Family: Upstate View All» Product Name: Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate | 16-103 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Biotin View All» Host: Mouse View All»
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