货号：14-921

品牌：Merck Millipore

规格：1EA

目录价：￥7525.80

市场价格：￥6396.93

会员价格：￥6020.64

金山科研平台，产品价格货期咨询微信：jinshanbio Description: CpG MethylQuest™ Protein (Recombinant GST-MBD fusion protein) View All» Qty/Pk: 100 µg View All» Product Overview: The CpG MethylQuest protein is an effective and versatile tool for the study of CpG methylation in DNA. This purified recombinant protein contains the methyl binding domain (MBD) of the mouseMBD2b protein fused to a glutathione-S-transferase protein (GST) from S japonicum. These two protein domains are separated by a linker containing a thrombin cleavage site.This unbound protein has several unique properties that can be incorporated into your own customized assays for the study of DNA methylation.

• Competitive elution of the GST-MBD protein or GST-MBD-methylated DNA complexes with glutathione.

• Elution of intact complexes by thrombin digestion

• Immobilization of protein DNA complexes or GST-MBD protein to other solid surfaces such as microtiter plates coated with GST antibodies.

• Visualization of complexes using labeled GST antibodies or GST antibodies and labeled secondary antibodies.

• Surface cysteine groups on the GST protein can be chemically modified to allow the addition of other reporters, affinity tags, or functional groups.
  To simplify the isolation of methylated DNA, this protein can also be obtained pre-bound to magnetic beadsas a component of the CpG MethylQuest DNA Isolation Kit (17-10035). View All» Key Applications: DNA Purification View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Source: GST-tagged full length recombinant methyl binding domain (MBD) of the mouse MBD2b protein expressed in E. coli View All» Presentation: Recombinant protein in buffer containing 20 mM HEPES pH 7.4, 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50% (v/v) glycerol. View All» Storage Conditions: 6 months at -20°C from date of receipt. For maximum activity protect the protein from temperature shifts. View All» Product Name: CpG MethylQuest™ Protein View All» Alternate Names:
  • Methyl Binding domain protein 2b

  • MBD2b

  • Methyl CpG binding domain protein
    View All» Concentration: 1 mg/mL View All» Quality Assurance: Functional Binding Assay: CpG MethylQuest protein bound to magnetic beads by the GST domain is used to isolate a methylated SNRPN DNA fragment from HeLa genomic DNA. View All» Purity: ~95% by SDS Page View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://16ao.com/thread-147061.htm
    © Merck Millipore代理14-921 CpG MethylQuest(R)Protein (Recombinant GST-MBD fusion protein), 100 µg，产品报价联系微信jinshanbio