货号：14-468

品牌：Merck Millipore

规格：

目录价：￥9278.00

市场价格：￥7886.30

会员价格：￥7422.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Crk (120-225)-GST View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 500 µg View All» Product Overview: Recombinant fusion protein containing amino acids 120-225 of mouse Crk with an N-terminal GST-tag View All» Key Applications: Kinase Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Entrez Gene Summary: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. View All» UniProt Summary: FUNCTION: SwissProt: P46108 # The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk- II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4.SIZE: 304 amino acids; 33831 Da SUBUNIT: Interacts with ABL1, C3G, SOS, MAP4K1, MAPK8 and DOCK3 via its first SH3 domain. Interacts with BCAR1, CBL, CBLB, PXN and GAB1 via its SH2 domain upon stimulus-induced tyrosine phosphorylation. Interacts with several tyrosine-phosphorylated growth factor receptors such as EGFR, PDGFR and INSR via its SH2 domain (By similarity). Interacts with DOCK1 and DOCK4. Interacts with SHB.SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell membrane (By similarity). Note=Translocated to the plasma membrane upon cell adhesion (By similarity).DOMAIN: SwissProt: P46108 The C-terminal SH3 domain function as a negative modulator for transformation and the N-terminal SH3 domain appears to function as a positive regulator for transformation (By similarity). & The SH2 domain mediates interaction with SHB.PTM: Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. & Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway.SIMILARITY: Contains 1 SH2 domain. & Contains 2 SH3 domains. View All» Species: Mouse View All» Molecular Weight: 37kDa View All» Brand Family: Upstate View All» Source: expressed in E. coli View All» Protein Target: Crk View All» Storage Conditions: 1 year at -20°C View All» Target Sub-Family: CMGC View All» Gene Symbol:

• CRK

• p38

• CRKII
  View All» Product Name: Crk (120-225)-GST View All» Quality Assurance: routinely evaluated by phosphorylation of Crk (120-225)-GST View All» UniProt Number: P46108 View All» Specific Activity: For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme. View All» Entrez Gene Number:
  • NM_005206.3

  • NM_016823.2
    View All» Purification Method: Glutathione Sepharose™ View All»
    © 金山科研平台是专业的授权总代理区域代理经销平台。
    © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
    © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
    © 本文地址：https://16ao.com/thread-140802.htm
    © Merck Millipore代理14-468 Crk (120-225)-GST (recombprotein expressed in E. coli)，产品报价联系微信jinshanbio