货号：14-1047

品牌：Merck Millipore

规格：

目录价：￥2647.00

市场价格：￥2249.95

会员价格：￥2117.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: 5-hmC Glucosyltransferase (β- glucosyltransferase) View All» Trade Name: Upstate (Millipore) View All» Qty/Pk: 200 Units View All» Product Overview: Specific glycosylation of 5-hydroxymethylcytosine (5-hmC)5-hmC Glucosyltransferase catalyzes the transfer of a glucose moiety from uridine diphosphoglucose (UDPG) to existing 5-hmC in DNA to form glucosyl-5-hydroxymethylcytosine (glucosyl-5-hmC). 5-hmC Glucosyltransferase does not modify cytosines or methylated cytosines (5-mC). Glucosylation of 5-hmC can be used for sequence-specific, locus-specific, or global quantification of 5-hmC.Validation: 5-hmC Glucosyltransferase was incubated with three different DNA standards from the CpGenome™ 5-hmC and 5-mC DNA Standard Set (Cat. No. S8005), containing either 5-hmC, 5-mC, or no cytosine modifications. After glucosylation, the Standards were treated with CviQ1, a selective restriction endonuclease, which cleaves unmethylated, methylated, and hydroxymethylated DNA, but not glucosylated 5-hmC DNA. Agarose gel analysis of restriction digests showed a single band (undigested) only for the glycosylated 5-hmC Standard. View All» Key Applications: Enzyme Assay View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Components:

• 5-hmC Glucosyltransferase Enzyme; 200 Units

• 10X Glucosyltransferase Reaction Buffer; 1mL

• 10X Uridine Diphosphoglucose; 300 µL
  View All» Brand Family: Upstate View All» Presentation: Single box containing three reagents necessary for a standard 5-hmC glycosyltransferase reaction: a highly active 5-hmC Glucosyltransferase enzyme, 10X Glucosyltransferase Reaction Buffer, and 10X Glucosyltransferase Reaction Buffer. View All» Storage Conditions: Store at -20ºC for up to 1 year. 5-hmC Glucosyltransferase can be stored for longer periods at -80ºC Avoid repeated freeze/thaw cycles. View All» Unit Definition: 5-hmC Glucosyltransferase is presented in a concentration of 2 Units/µL. One Unit is defined as the quantity of 5-hmC Glucosyltransferase required to glycosylate 1 µg of 5-hmC DNA Standard (Cat. No. S8005) and prevent Glal digestion in a reaction incubated for 1 hour at 30ºC. View All» Product Name: 5-hmC Glucosyltransferase (β- glucosyltransferase) View All» Materials Required but Not Delivered: 1.5 mL microcentrifuge tubesHeat block View All»
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