金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Leu34-Leu202, with an N-terminal 7-His tag Accession #:P09225 N-terminal Sequence Analysis:His Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:19.5 kDa SDS-PAGE:25 kDa, reducing conditions Activity:Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycinD. Matthews,N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED₅₀ for this effect is typically 0.125 - 0.5 µg/mL. Molecule Information: TNF-beta/TNFSF1B Long Name: Tumor Necrosis Factor beta Aliases: LTA; Lymphotoxin alpha; tnfb; tnf-b; TNFSF1; TNFSF1B Entrez Gene IDs: 4049 (Human); 16992 (Mouse) Background: TNF-beta/TNFSF1B/TNFSF1 TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNF-beta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.