金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Glu39-Thr479, with an N-terminal 6-His tag Accession #:AAH93690 N-terminal Sequence Analysis:His Purity:>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. Endotoxin Level: Predicted Molecular Mass:51 kDa SDS-PAGE:66-90 kDa, reducing conditions Activity:Measured by its ability to transfer sulfate from PAPS to chondroitin sulfate.The specific activity is >350 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: Carbohydrate Sulfotransferase 3/CHST3 Aliases: C6ST1; CHST3; GST-0 Entrez Gene IDs: 9469 (Human); 53374 (Mouse); 84468 (Rat) Background: Carbohydrate Sulfotransferases 3 The mouse CHST family is comprised of 13 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia. CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. Mouse CHST3 shares 85% amino acid sequence identity to the human ortholog.