金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived Accession #:P16581 N-terminal Sequence Analysis:Trp22 Structure:Disulfide-linked homodimer Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:86 kDa (monomer) SDS-PAGE:150 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.When 5 x 10⁴ cells/well are added to human E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 90 - 100% will adhere after 1 hour incubation at RT. Optimal dilutions should be determined by each laboratory for each application. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: E-Selectin/CD62E Aliases: CD62E; ELAM1; LECAM2; SELE Entrez Gene IDs: 6401 (Human); 20339 (Mouse); 25544 (Rat) Background: Selectins The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.