金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Leu31-Lys1403 with an N-terminal 6-His tag Accession #:NP_032721 N-terminal Sequence Analysis:Not obtained, predicted His-tag Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:151.5 kDa SDS-PAGE:170-185 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.When5 x 10⁴ cells/well are added toRecombinant Mouse Nidogen-2coated plates (10 µg/mL with 100µL/well), >40% will adhere after one hour at 37 °C. Optimal dilutions should be determined by each laboratory for each application. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: Nidogen-2 Aliases: NID2; Osteonidogen Entrez Gene IDs: 22795 (Human); 18074 (Mouse); 302248 (Rat) Background: Nidogens Nidogen-2 (also called entactin-2) is a 200 kDa, secreted, basement membrane glycoprotein. Nidogens-1 and -2 are expressed in nearly all basement membranes where they interact with laminins, collagen type IV, and proteoglycan family members. Nidogen-2 contains three globular domains (G1-3) separated by a link region and an extended rod-shaped segment. The G1 domain is reported to bind type IV collagen, the G2 domain interacts with perlecan, and C-terminal G3 is associated with laminin binding. There is one EGF-like motif and a short peptide that ligates alpha 3 beta 1 integrins. The rod-shaped segment contains four additional EGF-like motifs and two thyroglobulin type 1 domains that serve as a binding site for alpha V beta 3 integrins.