金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Glu23-Cys1321, with a C-terminal 6-His tag Accession #:O14594 N-terminal Sequence Analysis:Glu23 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:141.5 kDa SDS-PAGE:140-290 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells.When 5 x 10⁴ cells per well are added to recombinant human Neurocan coated plates, cell adhesionis enhanced in a dose dependent manner.The ED₅₀ for this effect is typically 50-200 ng/mL. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: Neurocan Aliases: CSPG3; NCAN; NEUR Entrez Gene IDs: 1463 (Human); 13004 (Mouse); 58982 (Rat) Background: Neurocan Neurocan, also known as CSPG3 and 1D1, is a secreted chondroitin sulfate proteoglycan that is primarily expressed in the central nervous system. Human Neurocan is predicted to be cleaved following Met635, resulting in N-terminal (Neurocan-130) and C-terminal (Neurocan-C) fragments. Neurocan and Neurocan-C are produced by astrocytes and accumulate in the matrix surrounding axonal bundles and neuronal cell bodies. Neurocan-130 is found mainly in the glial cell cytoplasm. Neurocan inhibits neuronal adhesion and neurite outgrowth through interactions with a variety of matrix and transmembrane molecules.