货号：2366P

品牌：Cell Signaling Technology (CST)

规格：40µl

目录价：￥1764.00

市场价格：￥1411.20

会员价格：￥1375.92

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Applications	Dilution	Species-Reactivity	Sensitivity	MW (kDa)	Isotype
W	1:1000	All Species Expected	Endogenous		Mouse IgG1
IP	1:200

Species cross-reactivity is determined by western blot.

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Specificity / Sensitivity

His-Tag (27E8) Mouse mAb detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.

His-Tag (27E8) Mouse mAb仅识别6xHis标签，此抗体可以识别转染细胞靶蛋白在氨基或羧基末端融合的6xHis-tag 。

Source / Purification

Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.

单克隆抗体是用含有流感血凝素表位(YPYDVPDYA的合成肽段肽段免疫动物生产的。

Western Blotting

Western blot analysis of extracts from control (lane 1) or cells expressing C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb.Weatern blot分析对照组细胞提取物（泳道1），泳道2 为C末端表达His标签的蛋白，所用抗体为His-Tag (27E8) Mouse mAb. 。

IP

Immunoprecipitation of control lysate (lane 1) or C-terminal His-tagged protein (lane 2), using His-Tag (27E8) Mouse mAb, followed by Western analysis with His-Tag Polyclonal Antibody #2365.免疫沉淀分析对照组细胞提取物（泳道1），泳道2 为C末端表达His标签的蛋白，所用抗体为His-Tag (27E8) Mouse mAb，采用His-Tag Polyclonal Antibody #2365进行western分析。

Background

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。

A variety of plasmids contain DNA that encodes an amino-terminal tag cosisting of six histidine (6xHis) residues followed by an extended multiple cloning site. The 6xHis tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography (1).As is the case with other protein tag systems (2), this polyhistidine tag can often be cleaved at sites recognized by proteases such as thrombin and enterokinases to isolate the protein of interest (1).

很多质粒包含一种DNA，可以编码氨基末端带有6个组氨酸（ 6xHis) ）残基，其后为延伸的多克隆结合位点（1）， 于其它蛋白标签系统一样（2），这种多组氨酸标签常在剪切位点被蛋白酶剪切，如凝血酵素和肠激酶，用于分离所需要的蛋白（1）。

1. Kroll, D.J. et al. (1993)DNA Cell Biol.12, 441-453.

2. di Guan, C. et al. (1988)Gene67, 21-30.

Application References

Rishi, A. K. et al. (2003)J. Biol. Chem.278, 33422-33435.Applications:IC-ABC,Western Blotting

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Protocols 2366 :Western Blotting Immunoprecipitation (Magnetic) Companion Products

12630 SignalFire™ Plus ECL Reagent

12757 SignalFire™ Elite ECL Reagent

2272 Myc-Tag Antibody

2276 Myc-Tag (9B11) Mouse mAb

2365 His-Tag Antibody

2367 HA-Tag (6E2) Mouse mAb

2372 β-Gal (14B7) Mouse mAb

2396 MBP Tag (8G1) Mouse mAb

6883 SignalFire™ ECL Reagent

7076 Anti-mouse IgG, HRP-linked Antibody

7727 Biotinylated Protein Ladder Detection Pack

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