金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Gln23-Lys277 + Gly1094-Leu1464, with an N-terminal human CD33 signal sequence and 6-His tag. Accession #:P02452 N-terminal Sequence Analysis:His &Asp1219 Purity:>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 µg per lane. Endotoxin Level: Predicted Molecular Mass:65 kDa (full-length), 40 kDa, 28 kDa SDS-PAGE:79-83 kDa, 54-56 kDa, 35-36 kDa, reducing conditions Activity:Measured by the cleavage of its C-terminal propeptide by Recombinant Human BMP‑1/PCP (Catalog# 1927-ZN).>50% of full-length Pro-CollagenI alpha 1is cleaved byRecombinant Human BMP‑1/PCP (Catalog# 1927-ZN) , as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: Collagen I alpha 1 Aliases: COL1A1; OI4 Entrez Gene IDs: 1277 (Human) Background: Collagen I alpha 1 Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a procollagen molecule which is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. The triple helical domain contains Gly-Xaa-Yaa triplets where Xaa and Yaa are frequently proline and hydroxyproline, respectively. The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues. Type I collagen is a heterotrimer that consists of two alpha 1(I) chains and one alpha 2(I) chain, although homotrimers consisting of three identical alpha 1(I) chains have also been described. This recombinant mini pro-alpha 1(I) collagen consists of a shortened alpha 1(I) chain with following domain structure from N- to C-terminus: N-propeptide, N-telopeptide, the 33 most N-terminal Gly-Xaa-Yaa repeats, the 33 most C-terminal Gly-Xaa-Yaa repeats, C-telopeptide and C-propeptide. The preparation contains a mixture of the full-length molecule, pN collagen I (alpha 1) and the C-terminal propeptide. This truncated pro-alpha 1(I) collagen is a substrate for procollagen N-proteinase and procollagen C-proteinase.