金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Met1-Asn627, with a C-terminal 6-His tag Accession #:Q14392 N-terminal Sequence Analysis:Ala18 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:67.0kDa (monomer) SDS-PAGE:70-80 kDa, reducing conditions Activity:Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells.Human PBMC cultured for 72 hours with PHA were incubated for an additional 3days in 96well plates coated with anti-CD3 and rhLRRC32.The ED₅₀ for this effect is typically 0.7 - 2.8 µg/mL.Optimal dilutions should be determined by each laboratory for each application. Measured by its binding ability in a functional ELISA.When rhLRRC32 is immobilized at 5 µg/mL (100 µL/well),rhLatent TGF-beta 1 (R&DSystems, Catalog# 299-LT)binds with an apparent K_D < 30 nM. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: LRRC32/GARP Long Name: Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant Aliases: D11S833E; GARP; Garpin Entrez Gene IDs: 2615 (Human); 434215 (Mouse) Background: TGF-beta Binding Proteins TGF-beta binding proteins 1-4/Latent TGF-beta binding proteins 1-4 (LTBPs 1-4) are members of the LTBP/fibrillin family of proteins. They are characterized by the presence of multiple EGF-like repeats and a series of cysteine-rich repeats. Extracellularly, LTBPs form part of a large inactive TGF-beta complex where they serve to anchor a mature, dimeric TGF-beta/TGF-beta propeptide complex to the ECM.