金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived Accession #:Q63722.2 N-terminal Sequence Analysis:Ser32 Structure:Disulfide-linked homodimer Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:141.7 kDa (monomer) SDS-PAGE:180 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842.The ED₅₀ for this effect is typically 0.5 - 2.0 µg/mL.Measured by its binding ability in a functional ELISA.Immobilized rrNotch-1 at 5µg/mL (100 µL/well) binds rrJagged-1 with an apparent K_D<10nM.Measured by the ability of the immobilized protein to inhibit C2C12 mouse myoblast cell myogenesis. Lindsell, C.E.et al. (1995) Cell 80:909; Varnum-Finney, B. et al. (2000) J. Cell Sci. 113:4313.When 1 x 10⁴ to 5 x 10⁴cells/well are added to Recombinant Human TROP‑2 Fc Chimera coated plates (10µg/mL with 100µL/well),>45%cells will adhere after 1hour incubation.Optimal dilutions should be determined by each laboratory for each application. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: Jagged 1 Aliases: CD339; JAG1 Entrez Gene IDs: 182 (Human); 29146 (Rat) Background: Jagged 1 and 2 Mammalian Jagged proteins are homologs of the Drosophila Notch ligand, Serrate, and together with Delta-like proteins, constitute ligands for the mammalian Notch proteins. Jagged 1 and 2 are 180 kDa and 150 kDa type I transmembrane glycoproteins, respectively, that possess extracellular DSL (delta, serrate, lag-2 consensus sequence) domains necessary for binding to Notch receptors, along with 15-16 calcium-binding EGF-like repeats which modulate the binding affinity. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis and development of the vasculature. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for functional Notch signaling.