金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Asn18-Leu274, with a C-terminal 6-His tag Accession #:AAA27789 N-terminal Sequence Analysis:Asn18 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:30 kDa SDS-PAGE:55-65 kDa, reducing conditions Activity:Measured by its ability to inhibit the adhesion of PMA-activated human neutrophils.The ED₅₀ for this effect is typically 0.1-0.4 µg/mL.Also measured by its ability to bind Recombinant Human Integrin alpha M beta 2 (Catalog# 4047-AM) in a functional ELISA. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS, NaCl, and DTT.See Certificate of Analysis for details. Molecule Information: NIF Long Name: Neutrophil Inhibitory Factor Background: NIF Neutrophil inhibitory factor (NIF) of the dog hookworm, Ancylostoma caninum, is a 41 kDa secreted glycoprotein that is thought to allow the hookworm to evade the innate immune defenses of the host. NIF interacts with the leukocyte integrin alpha M beta 2 (CD11b/CD18, also called Mac-1), blocking adhesion to ligands including ICAM-1/CD54, complement component C3b, and fibrinogen, and effectively inhibiting neutrophil adhesion to endothelium. In mammalian inflammatory lung injury models, administration of NIF prevented neutrophil-dependent vascular injury.