金山科研平台，产品价格货期咨询微信：jinshanbio Source:E. coli-derived, Met1-Arg376, with Met, 6-His tag and a fusion partner at the N-terminus Accession #:NP_813586 N-terminal Sequence Analysis:Met Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:55 kDa SDS-PAGE:59 kDa, reducing conditions Activity:Measured by its ability to liberate oligosaccharides from heparin.The specific activity is >12,000 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl and Citrate.See Certificate of Analysis for details. Molecule Information: Heparinase I Entrez Gene IDs: 1073402 (B. thetaiotaomicron) Background: Heparinase I Heparin and heparan sulfate are sulfated glycosaminoglycans that share basic carbohydrate backbone structure with alternating uronic acid and N-acetylglucosamine residues. Heparin is found in mast cells and has strong anticoagulation properties. Heparan sulfate is found on cell membrane and extracellular matrix and is involved in various biological events from cell growth, adhesion and migration to lipid metabolism. Heparin has a much higher degree of sulfation than heparan sulfate, which can be considered as a polysaccharide with regions similar to heparin interspaced with much less sulfated regions. Both heparin and heparan sulfate can be digested by heparinases, a group of bacterial lyases that are widely used as tools for processing and analyze these polysaccharides. Heparinase I from Bacteroides thetaiotaomicron is a newly discovered heparinase with no activity against chondroitin sulfate and keratan sulfate. The enzyme readily releases tri-sulfated and di-sulfated disaccharides from heparin and heparan sulfate.