货号：09-890

品牌：Merck Millipore

规格：

目录价：￥5657.00

市场价格：￥4808.45

会员价格：￥4525.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-AMPylated Threonine Antibody | 09-890 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes AMPylated Ras-related C3 botulinum toxin substrate 1. View All» Molecular Weight: ~22 kDa observed. Other AMPylated proteins may appear at a different molecular weights. View All» Epitope: AMPylated Threonine View All» Immunogen: KLH-conjugated linear peptide corresponding to the Rac1 switch I region with AMPylated threonine-35 residue of AMPylated Ras-related C3 botulinum toxin substrate 1. View All» Background Information: The PTM AMPylation, previously known as adenylylation, is the covalent addition of an Adenosine MonoPhosphate group to a hydroxyl side chain of threonine, tyrosine, and potentially serine residues. AMPylation of the threonine residue on such GTPases as; Rho, Rac and Cdc42 could interfere with the interaction of effectors downstream and shut down actin assembly in an infected cell. This antibody against AMPylated threonine has great potential to help in the identification of proteins containing this posttranslational modification. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. Broad species reactivity expected. View All» Application Notes: Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Yi-Heng, H., et al. (2011). Journal of Biotechnology. 151:251–254.) View All» Control: HeLa cell lysate incubated with 10 ng GST-VopS in the presence of 0.5 mM ATP View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate incubated with 10 ng GST-VopS in the presence of 0.5 mM ATP.Western Blot Analysis: 1:2,000 dilution of this antibody detected AMPylated Threonine on 10 µg HeLa cell lysate incubated with 10 ng GST-VopS in the presence of 0.5 mM ATP. (Yi-Heng, H., et al. (2011). Journal of Biotechnology. 151:251–254.) View All» Purification Method: depletion View All» Presentation: Rabbit polyclonal depleted serum containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» Gene Symbol:

• RAC1

• TC25

• MIG5
  View All» Alternate Names:
  • AMPylated Threonine

  • Ras-related C3 botulinum toxin substrate 1

  • Cell migration-inducing gene 5 protein

  • Ras-like protein TC25

  • p21-Rac1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation
      View All» Product Name: Anti-AMPylated Threonine View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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