货号：09-813

品牌：Merck Millipore

规格：100Ug

目录价：￥4797.00

市场价格：￥4077.45

会员价格：￥3837.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RING finger protein 8 Antibody | 09-813 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes RING finger protein 8. View All» Molecular Weight: ~ 58 kDa observed. Two different isoforms have been described. Isoform 1: 55.5 kDa and Isoform 2: 11 kDa View All» Epitope: 0 View All» Immunogen: Linear peptide corresponding to amino acids 135-148 of human RING finger protein 8. View All» Background Information: RING Finger protein 8 (RNF8) is DNA binding protein belonging to the RING (Really Interesting New Gene) finger group of proteins. This family is characterized by a RING finger domain containing a Cys3HisCys4 amino acid motif capable of binding two zinc cations. RNF8 contains two domains of interest, the characteristic RING finger motif in the C-terminus and a FHA domain within the N-terminus, and is a critical E3 ubiquitin-protein ligase involved in the assembly of repair proteins to damaged DNA sites. After DNA double-strand breaks, RNF8 is recruited by ATM-phosphorylated MDC1 to the damage sites, where it functions to mediate H2A and H2AX ubiquitination, which in turn promotes TPR53BPI formation and the ionizing radiation-induced foci (IRIF) of BRCA1. RNF8 may also play regulatory roles in cytokinesis, correct exiting from mitosis, and RXRA-mediated transcriptional activity. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with pan trogloytes and macaca mulatta based on 100% sequence homology. View All» Application Notes: Western Blot (SNAP ID) Analysis: 1 µg/mL of a representative lot of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract.Immunocytochemistry Analysis: 1:500 dilution of a representative lot this antibody detected RING finger protein 8 in NIH/3T3 and A431 cells. View All» Control: U2OS nuclear extract View All» Quality Assurance: Evaluated by Western Blot in U2OS nuclear extract.Western Blot Analysis: 0.1 µg/mL of this antibody detected RING finger protein 8 on 10 µg of U2OS nuclear extract. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O76064 View All» Entrez Gene Number: NP_003949 View All» Gene Symbol: RNF8 View All» Alternate Names:
  • ring finger protein 8

  • C3HC4-type zinc finger protein

  • ring finger protein (C3HC4 type) 8

  • UBC13/UEV-interacting ring finger protein, E3 ubiquitin-protein ligase RNF8
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» Entrez Gene Summary: The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13 (UBE2N). Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBC13 but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2. PATHWAY: Protein modification; protein ubiquitination.SUBUNIT STRUCTURE: Interacts with class III E2s, including UBE2E1, UBE2E2, and UBE2E3 and with UBE2N. Interacts with RXRA. Interacts with ATM-phosphorylated MDC1. SUBCELLULAR LOCATION: Nucleus. Note: Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle. Ref.10 Ref.11 Ref.12 Ref.13 Ref.22 TISSUE SPECIFICTY: Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells. DEVELOPMENTAL STAGE: Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase. DOMAIN: The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1.PTM: Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger.SEQUENCE SIMILARITIES: Belongs to the RNF8 family.Contains 1 FHA domain.Contains 1 RING-type zinc finger.SEQUENCE CAUTION: The sequence BAA31621.2 differs from that shown. Reason: Erroneous initiation. The sequence BAG60572.1 differs from that shown. Reason: Erroneous translation. Wrong choice of CDS.The sequence EAX03945.1 differs from that shown. Reason: Erroneous gene model prediction. View All» Product Name: Anti-RING finger protein 8 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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