金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Pro28-Trp401, with an N-terminal 6-His tag Accession #:AAY14736 N-terminal Sequence Analysis:His Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:45 kDa SDS-PAGE:56-60 kDa, reducing conditions Activity:Measured by its ability to transfer sulfate from PAPS to heparan sulfate.The specific activity is >45 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl and EDTA.See Certificate of Analysis for details. Molecule Information: Heparan Sulfate 6-O-Sulfotransferase 1/HS6ST1 Entrez Gene IDs: 9394 (Human); 50785 (Mouse); 316325 (Rat) Background: HS6ST1 Heparan Sulfate is a highly sulfated polysaccharide that can be found on cell surface and extracellular matrix. It is usually covalently attached to a protein core as the glycan component of a proteoglycan. Heparan Sulfate interacts with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins, thus playing roles from cell growth, cell differentiation, cell motility, blood coagulation, lipid metabolism to viral infection. Heparan Sulfate consists of repeating residues of uronic acid and N-acetylglucosamine. The uronic acid residues can be sulfated at 2-O position by Heparan Sulfate 2-O Sulfotransferase (HS2ST). The N-acetylglucosamine residues can be sulfated at N-, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), Heparan Sulfate 3-O and 6-O sulfotransferases (HS3STs and HS6STs) respectively. However, the reactions catalyzed by these sulfotransferases are normally incomplete on the whole chain of Heparan Sulfate. As a result, Heparan Sulfate displays enormous sequence diversity that allows it to interact with a wide spectrum of proteins differently. Among three HS6STs, HS6ST1 is the first to be cloned. Mice deficient of the HS6ST1 homologue gene showed embryonic lethality.