货号：07-870

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-PKCβ I&II (Thr500) Antibody | 07-870 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes phosphorylated PKCβ I&II (Thr500). Minor cross reaction can occur with PKCα (Thr497), PKCγ (Thr514), and PKCε (Thr566) dependent upon cell type and treatment. View All» Molecular Weight: ~80 kDa View All» Immunogen: Peptide corresponding to amino acid region encompassing the human phospho-PKCβ I&II (Thr500) View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Species Reactivity: Human View All» Species Reactivity Note: Predicted to cross-react with mouse and rat based sequence homology, but have not been tested. View All» Application Notes: Immunoblot Analysis: A 1:1000 dilution of this lot detected PKCβ I&II in RIPA lysates from PMA treated K562 cells. View All» Quality Assurance: Routinely evaluated by immunoblot. View All» Purification Method: Affinity Purfied View All» Presentation: 100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 1.0 mg/mL BSA (IgG, protease free) and 0.05% sodium azide) and 50% glycerol. View All» Storage Conditions: Stable for 2 years at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P05771 View All» Entrez Gene Number:

• NM_002738.5

• NM_212535.1
  View All» Gene Symbol:
  • PRKCB1

  • PRKCB

  • PRKCB2

  • MGC41878

  • PKCB

  • PKC-B

  • PKC-beta
    View All» Alternate Names: PRKCB1, PRKCB2, PKCB, PKC beta, protein kinase C, beta 1&2 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. View All» UniProt Summary: FUNCTION: SwissProt: P05771 # This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May be considered as a novel component of the NF-kappa-B signaling axis responsible for the survival and activation of B-cells after BCR cross-linking (By similarity).COFACTOR: Binds 3 calcium ions per subunit. The ions are bound to the C2 domain (By similarity).SIZE: 671 amino acids; 76869 Da SUBUNIT: Interacts with PDK1 (By similarity). Interacts in vitro with PRKCBP1.SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).PTM: Phosphorylation on Thr-500 of isoform beta-I, within the activation loop, renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Similarly, isoform beta-II is autophosphorylated on 'Thr-640' and 'Ser-659', subsequent to phosphorylation on Thr-500. Autophosphorylated on other sites i.e. in the N-terminal and hinge regions have no effect on PKC activity (By similarity).SIMILARITY: SwissProt: P05771 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-PKCβ I&II (Thr500) Antibody | 07-870 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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