货号：07-428

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-eNOS (Ser1177) Antibody | 07-428 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: Note: ADMA (Catalog #19-169) and NMMA (Catalog #19-170) are inhibitors of NOS View All» Specificity: Recognizes phosphorylated eNOS, Mr 132 kDa. Additional unknown bands at 50 and 64 kDa were competed by the immunizing peptide. View All» Molecular Weight: 132 kDa View All» Epitope: Ser1177 View All» Immunogen: KLH-conjugated, synthetic peptide (C-RIRTQ[pS]FSLQER) corresponding to amino acids 1172-1183 of human eNOS (endothelial nitric oxide synthase) with an N-terminal cysteine added for conjugation purposes. The immunizing sequence is identical in rat, mouse, bovine, pig, and dog. View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: NO, produced by nitric oxide synthases (NOS), is a critical signaling molecule involved in many physiological processes including muscle contraction, vasodilation, neuronal transmission, and immune responses. Some of these effects are mediated through reaction with and activation of guanylate cyclase (GC), resulting in increased cGMP production. With a half-life of seconds, NO readily diffuses through the cytosol and cell membrane, allowing it to act inside or adjacent to the cell in which it was produced. View All» Species Reactivity:

• Human

• Mouse

• Rat

• Bovine

• Canine
  View All» Species Reactivity Note: Human; other species not tested. Predicted to cross-react with mouse, rat, cow, and dog based on sequence homology. View All» Control: Brain tissue, VEGF treated HUVEC cell lysate. View All» Quality Assurance: Routinely evaluated by western blot from VEGF treated HUVEC cell lysates.Western Blot Analysis: A 1:1000 dilution of this lot detected recombinant eNOS phosphorylated by PKA and endogenous eNOS from VEGF treated HUVEC cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal antiserum IgG in buffer containing 0.05% sodium azide and glycerol to 30%. Liquid at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P29474 View All» Entrez Gene Number: NP_000594 View All» Gene Symbol:
  • NOS3

  • cNOS

  • ECNOS

  • eNOS

  • EC-NOS

  • NOSIII
    View All» Alternate Names:
    • Constitutive NOS

    • Endothelial NOS

    • Endothelial NOS

    • Endothelial NOS

    • endothelial nitric oxide synthase

    • nitric oxide synthase 3 (endothelial cell)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: SwissProt: P29474 # Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. No mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.COFACTOR: Heme group. & Binds 1 FAD. & Binds 1 FMN. & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme.SIZE:1203 amino acids; 133289 Da SUBUNIT: Homodimer. Interacts with NOSIP and NOSTRIN.SUBCELLULAR LOCATION:Cell membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.TISSUE SPECIFICITY: Platelets, placenta, liver and kidney.SIMILARITY: SwissProt: P29474 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-eNOS (Ser1177) Antibody | 07-428 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Serum View All» Host: Rabbit View All»
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