金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Asp18-Lys505, with a C-terminal 10-His tag Accession #:P07237 N-terminal Sequence Analysis:Asp18 Purity:>80%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:56 kDa SDS-PAGE:60 kDa, reducing conditions Activity:Measured by its ability to promote aggregation of insulin in the presence of DTT. The specific activity is >7.5 A₆₅₀/min/mg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, NaCl and Glycerol.See Certificate of Analysis for details. Molecule Information: Protein Disulfide Isomerase/P4HB Long Name: Proline 4-Hydroxylase, Beta Polypeptide Aliases: DSI; ERBA2L; GIT; PDI; PDIA1; PO4DB; PO4HB; PROHB; Thbp Entrez Gene IDs: 5034 (Human); 18453 (Mouse); 25506 (Rat) Background: P4HB P4HB (Prolyl 4-hydroxylase beta chain; also PDI) is a 60 kDa member of the protein disulfide isomerase family. As an intracellular homodimer, it forms a tetrameric complex with P4H alpha chains to form an active prolyl 4 hydroxylase. This catalyses the hydroxylation of proline in collagen. On the cell surface, it reduces disulfide bonds in HIV that allow the virus to fuse with CXCR4 and enter susceptible cells. Mature human P4HB is 491 amino acids (aa) in length. It contains two TRX domains (aa 25-134 and 368-475) plus an ER retention sequence (aa 505-508). There is one potential isoform that shows an 11 aa substitution for the first 162 amino acids. Over aa 18-505, human P4HB shares 94% aa identity with mouse P4HB.