货号：07-247

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-IRS1 (Ser307) Antibody | 07-247 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes phospho-IRS1 (Ser307), MW ~170 kDa. An additional protein may be detected at MW ~110 kDa. View All» Molecular Weight: 170 kDa View All» Epitope: a.a. 301-315 View All» Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 301-315 (ESITATpSPASMVGGK) of phospho-IRS1 (Ser307) The immunizing sequence is identical in mouse, human, rat, and monkey and has 12/15 identical amino acids in chicken. View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: Insulin receptor substrate (IRS) molecules are key mediators in insulin signaling and play a central role in maintaining basic cellular functions such as growth, survival, and metabolism. They act as docking proteins between the insulin receptor and a complex network of intracellular signaling molecules containing Src homology 2 (SH2) domains. Four members (IRS-1, IRS-2, IRS-3, IRS-4) of this family have been identified that differ as to tissue distribution, subcellular localization, developmental expression, binding to the insulin receptor, and interaction with SH2 domain-containing proteins. Results from targeted disruption of the IRS genes in mice have provided important clues to the functional differences among these related molecules, suggesting they play different and specific roles in vivo. The available data are consistent with the notion that IRS-1 and IRS-2 are not functionally interchangeable in tissues that are responsible for glucose production (liver), glucose uptake (skeletal muscle and adipose tissue), and insulin production (pancreatic β cells). In fact, IRS-1 appears to have its major role in skeletal muscle whereas IRS-2 appears to regulate hepatic insulin action as well as pancreatic β cell development and survival. By contrast, IRS-3 and IRS-4 genes appear to play a redundant role in the IRS signaling system. Defects in muscle IRS-1 expression and function have been reported in insulin-resistant states such as obesity and type 2 diabetes. View All» Species Reactivity:

• Human

• Rat

• Monkey

• Mouse
  View All» Species Reactivity Note: Human, mouse, and rat. Predicted to cross-react with monkey based on sequence homology. View All» Control: MCF7 + insulin cell lysate, NIH/3T3 + anisomycin cell lysate. View All» Quality Assurance: Evaluated by western blot on RIPA lysates from serum starved HEK 293 cells treated with anisomycin.Western Blot Analysis: A 1:1000-1:2000 dilution of this antibody detected phospho-IRS1 (Ser307) in lysates from serum starved HEK 293 cells treated with 5 μg/mL anisomycin for 30 minutes, but not in untreated cells. View All» Purification Method: Immunodepleted Serum View All» Presentation: Rabbit polyclonal antiserum in TBS containing 0.05% sodium azide before the addition of glycerol to 30%. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: P35568 View All» Entrez Gene Number: NM_005544 (H) View All» Gene Symbol:
  • IRS1

  • IRS-1

  • HIRS-1
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: SwissProt: P35568 # May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit (By similarity).SIZE: 1242 amino acids; 131591 Da SUBUNIT: Interacts with the NPXY motif of tyrosine-phosphorylated IGF1R and INSR via the PTB domain. Binds to phosphatidylinositol 3-kinase p85 subunit via the phosphorylated YXXM motifs. Binds ROCK1. Binds to UBTF and PIK3CA in nuclear extracts (By similarity). Interacts with SOCS7.PTM: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor (By similarity). & Phosphorylation of Tyr-896 is required for GRB2-binding (By similarity).DISEASE:SwissProt: P35568 # Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853].SIMILARITY: SwissProt: P35568 ## Contains 1 IRS-type PTB domain. & Contains 1 PH domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-IRS1 (Ser307) Antibody | 07-247 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Serum View All» Host: Rabbit View All»
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