货号：07-191

品牌：Merck Millipore

规格：

目录价：￥5141.00

市场价格：￥4369.85

会员价格：￥4112.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Histone H2B (Ser14) Antibody | 07-191 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes phospho-Histone H2B (Ser14), Mr 15 kDa. View All» Molecular Weight: 15 kDa View All» Epitope: Ser14 View All» Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 6-16 of human histone H2B (C-SAPAPKKG[pS]KK). View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various posttranslational modifications, including acetylation, phosphorylation, methylation and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, on gene expression. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. Broad species cross-reactivity expected based on sequence homology. View All» Control: Acid extracted HL-60 cells treated with VP16 View All» Quality Assurance: Routinely evaluated by western blot on acid extracted HL-60 cells treated with VP16.Western Blot Analysis: 1:1,000-1:10,000 dilutions of this lot detected phospho-Histone H2B in acid extracted HL-60 cells treated with 20μg/mL VP16 for 18 hours. A 1:250-1:2,000 dilution of a previous lot also detected phospho-Histone H2B acid extracted proteins from colcemid-arrested HeLa cells.Note: The optimal antibody dilution of this antibody must be empirically determined. Use of this antibody at dilutions which are too low, will result in non-specific detection of Histone H2B and other unknown proteins in western blots of acid extracts from untreated cells. View All» Purification Method: Unpurified View All» Presentation: Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide and 30% glycerol. Liquid at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q8N257 View All» Entrez Gene Number: NP_778225 View All» Gene Symbol: HIST3H2BB View All» Alternate Names:

• H2BS14P

• Histone H2B (phospho S14)

• H2B type 12

• histone 3, H2bb

• histone H2B

• v
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • ELISA

  • Immunocytochemistry

  • Immunohistochemistry

  • Western Blotting
    View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. View All» UniProt Summary: FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 126 amino acids; 13908 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Histone H2B (Ser14) Antibody | 07-191 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Serum View All» Host: Rabbit View All»
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