金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Phe17-Gln317, with a C-terminal 10-His tag Accession #:AAC95490 N-terminal Sequence Analysis:Phe17 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:34.7 kDa SDS-PAGE:75-90 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of the MCF‑7 human breast cancer cells.When 5x10⁴ cells/well are added to recombinant human IBSP-coated plates (3µg/mL with 100µL/well), approximately 60-80% will adhere after 30minutes at 37°C.Optimal concentration depends on cell type as well as the application or research objectives. Formulation:Lyophilized from a 0.2 µm filtered solution in MES and NaCl.See Certificate of Analysis for details. Molecule Information: IBSP/Sialoprotein II Long Name: Integrin-binding Sialoprotein Aliases: BNSP; BSP-II; SP-II Entrez Gene IDs: 3381 (Human); 15891 (Mouse); 24477 (Rat) Background: IBSP

IBSP (integrin-binding sialoprotein; also BSP or bone sialoprotein II) is a 55 - 75 kDa, secreted, variably glycosylated, monomeric noncollagenous member of the SIBLING family of extracellular matrix (ECM) proteins. It is principally associated with the early stages of bone mineralization. IBSP is synthesized as a 317 amino acid (aa) precursor that contains a 16 aa signal sequence and a 301 aa mature region. The mature segment is divided into a basic N-terminus (aa 17 - 62), a central region (aa 63 - 233), and an acidic C-terminus (aa 234 - 317).

HAp formation requires a IBSP nucleation site composed of at least eight consecutive glutamic acid residues and, likely, a contribution from a BSP-associated co-nucleator. IBSP is highly glycosylated, sulfated, and phosphorylated. Phosphorylation may impact HAp growth, while carbohydrate may regulate cell adhesion. Mature human IBSP is 70%, 72%, 78%, and 72% aa identical to porcine, rat, canine, and mouse IBSP, respectively. IBSP is synthesized by megakaryocytes/platelets, osteoblasts, osteocytes, odontoblasts, osteoclasts, and bone marrow stromal cells.