货号：07-1719

品牌：Merck Millipore

规格：100Ug

目录价：￥5513.00

市场价格：￥4686.05

会员价格：￥4410.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-FOXO3A Antibody | 07-1719 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes FOXO3A. View All» Molecular Weight: ~80 kDa View All» Immunogen: Synthetic peptide corresponding to FOX03A View All» Background Information: Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Tested on human. Predicted to react with mouse based on sequence homology. View All» Application Notes: Immunocytochemistry: A 1:500 dilution of this antibody was used to detect FOXO3A in HeLa cells. Positive staining in the nucleus. View All» Control: FOXO3A in Jurkat cell lysate. View All» Quality Assurance: Western Blot Analysis: 1:500 dilution of this antibody was used to detect FOXO3A in Jurkat cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. View All» Storage Conditions: Stable for 1 year at 2-8ºC from date of receipt.To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses. View All» UniProt Number: O43524 View All» Entrez Gene Number: NP_001446 View All» Gene Symbol:
  • AF6q21

  • DKFZp781A0677

  • FKHRL1

  • FKHRL1P2

  • FOXO2

  • FOXO3A

  • MGC12739

  • MGC31925

  • OTTHUMP00000016944
    View All» Alternate Names:
    • AF6q21 protein

    • Forkhead in rhabdomyosarcoma-like 1

    • forkhead box O3

    • forkhead box O3A

    • forkhead homolog (rhabdomyosarcoma) like 1

    • forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry
        View All» Entrez Gene Summary: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]. View All» UniProt Summary: FUNCTION:Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Ref.5 Ref.7SUBUNIT: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. Ref.7SUBCELLULAR LOCATION:Cytoplasm › cytosol. Nucleus. Note= Translocates to the nucleus upon oxidative stress and in the absence of survival factors. Ref.5 Ref.7TISSUE SPECIFICITY: Ubiquitous. Ref.1PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. Ref.5 Ref.7 Ref.6 Ref.8 Ref.9DISEASE: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX.SIMILARITY: Contains 1 fork-head DNA-binding domain. View All» Product Name: Anti-FOXO3A Antibody (07-1719) View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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