货号：07-1643

品牌：Merck Millipore

规格：100Ug

目录价：￥3724.00

市场价格：￥3165.40

会员价格：￥2979.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Akt (Tyr450) Antibody | 07-1643 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes phosphorylated Akt at Tyr450. View All» Molecular Weight: Approx. 56 kDa View All» Epitope: Phosphorylated Tyr450 View All» Immunogen: KLH-conjugated linear peptide corresponding to human phosphorylated AKT at and around Tyr450. View All» Modifications: Phosphorylation View All» Background Information: Akt (protein kinase B), a serine/threonine kinase, has emerged as a critical enzyme in signal transduction pathways involved in cell proliferation, apoptosis, angiogenesis, and diabetes. In mammals three isoforms of Akt (α, β, γ or Akt 1, 2, 3) are reported that exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. Akt α is the predominant isoform in most tissues, whereas the highest expression of Akt β is observed in the insulin-responsive tissues, and Akt γ is abundant in brain tissue. Each Akt isoform is composed of three functionally distinct regions: an N-terminal pleckstrin homology (PH) domain that provides a lipid-binding module to direct Akt to PIP2 and PIP3, a central catalytic domain, and a C-terminal hydrophobic motif. View All» Species Reactivity:

• Mouse

• Rat

• Human

• Bovine

• Chicken

• Horse
  View All» Species Reactivity Note: Reacts with human, mouse, and rat. Predicted to react with cow, chicken, and horse based on sequence homology. View All» Application Notes: Western Blot (SNAP ID) Analysis: 1:1,000 dilution from a previous lot detected AKT phosphorylated at Tyr450 in NIH/3T3 cell lysate. View All» Control: Lambda phosphatase treated and untreated NIH/3T3 with Anisomycin, RAT2 with SRC, and MCF7 with EGF View All» Quality Assurance: Evaluated by Western Blot in NIH/3T3, RAT2, and MCF7 cell lysates.Western Blot Analysis: 1:250-1,000 dilution of this antibody detected AKT phosphorylated at Tyr450 in NIH/3T3, RAT2, and MCF7 cell lysates. View All» Purification Method: Affinity Purfied View All» Presentation: Affinity purified rabbit serum in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P31749 View All» Entrez Gene Number: NP_005154 View All» Gene Symbol:
  • AKT1

  • RAC

  • PRKBA

  • RAC-ALPHA

  • RAC-PK-alpha

  • C-AKT

  • PKB

  • AKT
    View All» Alternate Names:
    • AKT1 kinase

    • Protein kinase B

    • RAC-alpha serine/threonine-protein kinase

    • rac protein kinase alpha

    • v-akt murine thymoma viral oncogene homolog 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Enzyme Assay
        View All» Entrez Gene Summary: The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq] View All» UniProt Summary: FUNCTION: General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.SIZE: 480 amino acids; 55686 Da SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).TISSUE SPECIFICITY: In all human cell types so far analyzed.DOMAIN: Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain. View All» Product Name: Anti-phospho-Akt (Tyr450) View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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