货号：07-1415

品牌：Merck Millipore

规格：EA

目录价：￥3310.00

市场价格：￥2813.50

会员价格：￥2648.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-mTOR (catalystic domain) Antibody | 07-1415 View All» Replaces: 07-1415 is a recommended replacement for AB3882 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Recognizes mTOR (mammalian target of rapamycin). Tested reactivity includes human and rat.. View All» Molecular Weight: Recognizes at 289 kDa band on reducing gels. View All» Epitope: 1223-1290 aa mTOR View All» Immunogen: Recombinant GST fusion protein corresponding to amino acids 1223-1290 of human mTOR. View All» Isotype: IgG View All» Background Information: mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GbL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes. View All» Species Reactivity:

• Human

• Rat
  View All» Species Reactivity Note: Reacts with Human and Rat. View All» Control: L6 Cell Lysate View All» Quality Assurance: Routinely evaluated by Western Blot on L6 lysates.Western Blot Analysis: 1:500 dilution of this lot detected mTOR on 10 μg of L6 lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal IgG in PBS containing 10% glycerol with 0.02% sodium azide added as a preservative. View All» Storage Conditions: Stable at -20ºC in undiluted aliquots for up to one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P42345 View All» Entrez Gene Number: NM_004958.2 View All» Gene Symbol:
  • FRAP1

  • RAPT1

  • FLJ44809

  • MTOR

  • FRAP

  • mTOR

  • FRAP2

  • RAFT1
    View All» Alternate Names:
    • Mammalian target of rapamycin

    • FKBP-rapamycin associated protein

    • FK506 binding protein 12-rapamycin associated protein

    • FKBP-rapamycin associated protein
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene. [provided by RefSeq] View All» UniProt Summary: FUNCTION: Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex. TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis. SIMILARITY: Belongs to the PI3/PI4-kinase family. SIMILARITY: Contains 1 FAT domain. SIMILARITY: Contains 1 FATC domain. SIMILARITY: Contains 7 HEAT repeats. SIMILARITY: Contains 1 PI3K/PI4K domain. SEQUENCE CAUTION: Sequence=AAC39933.1; Type=Frameshift; Positions=956, 999; View All» Product Name: Anti-mTOR (catalystic domain) Antibody | 07-1415 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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