货号：07-049

品牌：Merck Millipore

规格：200UL

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Nedd4 Antibody, WW2 domain | 07-049 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Nedd4, Mr 115 kDa. Degradation products were also detected at Mr 55 and 60 kDa. View All» Molecular Weight: Approx. 115 kDa View All» Immunogen: GST fusion protein corresponding to the WW2 domain (residues 395-462) of rat Nedd4. View All» Isotype: IgG View All» Background Information: Neuronal precursor cell-expressed developmentally downregulated 4 (Nedd4) is an ~120 kDa E3 ubiquitin ligase. Nedd4 is involved in regulation of ubitiquin-targeted degradation of proteins. Nedd4 interacts with many different E2 enzymes, including human Ubc4, UbcH5B, UbcH5C, UbcH6 and UbcH7, suggesting it plays a role in multiple E2 ubiquitination pathways. Nedd4 contains a C2 domain and several WW domains, which recognize membrane surfaces and Pro-Tyr motifs, respectively. Nedd4 polyubiquitinates the epithelial sodium channel, and mutations that disrupt association between Nedd4 and the channel lead to Liddle’s syndrome, a hyperaldosteronism-like, hypertension disease. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse, and rat. View All» Application Notes: Immunohistochemistry: This antibody has been reported by an independent laboratory to detect Nedd4 in mouse kidney, lung, and distal colon sections. View All» Control: Kidney cytoplasmic extracts. View All» Quality Assurance: Routinely evaluated by Western Blot on Mouse Kidney Cytosol lysate.Western Blot Analysis: 1:10,000 dilution of this lot detected Nedd4, WW2 domain on 10 µg of Mouse Kidney Cytosol. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl and 0.05% sodium azide before the addition of glycerol to 30%. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P46934 View All» Entrez Gene Number: NP_006145.2 View All» Gene Symbol:
  • EC 6.3.2.-

  • KIAA0093

  • MGC176705

  • NEDD-4

  • NEDD4-1

  • RPF1
    View All» Alternate Names:
    • Neural precursor cell expressed developmentally

    • down-regulated protein 4

    • neural precursor cell expressed, developmentally

    • down-regulated 4

    • receptor-potentiating factor 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry

      • Western Blotting
        View All» UniProt Summary: FUNCTION: SwissProt: P46934 # E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (By similarity). Involved in the budding of many viruses.SIZE: 1000 amino acids; 114936 Da SUBUNIT: Interacts with UBE2D2. Binds SCNN1A, SCNN1B and SCNN1G. Binds, in vitro, through the WW2 and WW3 domains, to neural isoforms of ENAH that contain the PPSY motif. Interacts with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity). Interacts with viral proteins that contain a late- budding motif P-P-P-Y. This interaction is essential for viral particle budding of a lot of retroviruses, like HTLV-1 Gag and MLV Gag.SUBCELLULAR LOCATION: Cytoplasm (By similarity).DOMAIN: SwissProt: P46934 The WW domains mediate interaction with LITAF, RNF11, WBP1, WBP2, TMEPAI, NDFIP1 and PRRG2 (By similarity).SIMILARITY: Contains 1 C2 domain. & Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain. & Contains 4 WW domains. MISCELLANEOUS: A cysteine residue is required for ubiquitin- thioester formation. View All» Brand Family: Upstate View All» Product Name: Anti-Nedd4, WW2 domain View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Purified View All» Host: Rabbit View All»
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