货号：06-896

品牌：Merck Millipore

规格：200Ug

目录价：￥6742.00

市场价格：￥5730.70

会员价格：￥5393.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GFP (Green Fluorescent Protein) Antibody | 06-896 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes green fluorescent protein (GFP), Mr 30 kDa, and GFP-fusion proteins. View All» Molecular Weight: 30 kDa View All» Immunogen: His-tagged green fluorescent protein of Aequorea victoria. View All» Isotype: IgY View All» Background Information: Green fluorescent protein (GFP) is a 29 kDa fluorescent protein isolated from the jellyfish Aequorea victoria. Upon exposure to blue light (488 nm), GFP strongly fluoresces (Emission max: 507 nm). GFP can be fused to proteins without significantly interfering with their assembly and function, making GFP a powerful tool for monitoring gene expression and protein localization in living cells. View All» Species Reactivity: Vertebrates View All» Species Reactivity Note: Vertebrates View All» Application Notes: Immunocytochemistry: This antibody was reported to show positive immunostaining in cells transfected with an expression vector encoding Green Fluorescent Protein. View All» Control: Rat brain lysate, cells expressing GFP protein. View All» Quality Assurance: Routinely evaluated by Western Blot on rat brain lysate containing purified GFP (Catalog #14-392) or a GFP-fusion protein from a transfected E. coli cell lysate.Western Blot Analysis:0.5-2 μg/mL of this lot detected Green Fluorescent Protein from 50 μg of rat brain lysate containing 25 ng of purified Green Fluorescent Protein (Catalog # 14-392). A previous lot of this antibody also detected a GFP-fusion protein from a transfected E. coli cell lysate. View All» Purification Method: Ammonium Sulfate Precipitation and PEG View All» Presentation: Purified chicken polyclonal IgY from egg yolk in buffer containing 70% storage buffer (0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. View All» UniProt Number: P42212 View All» Gene Symbol: GFP View All» Alternate Names: Green fluorescent protein View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunocytochemistry

• Western Blotting
  View All» UniProt Summary: FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. BIOPHYSICOCHEMICAL PROPERTIES:Absorption: Abs(max)=395 nm; Note=Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm; SUBUNIT: Monomer. TISSUE SPECIFICITY: Photocytes. PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen. BIOTECHNOLOGY: Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. BIOTECHNOLOGY: Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy. SIMILARITY: Belongs to the GFP family. View All» Brand Family: Upstate View All» Product Name: Anti-GFP (Green Fluorescent Protein) Antibody | 06-896 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Chicken View All»
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  © Merck Millipore代理06-896 Anti-GFP (Green Fluorescent Protein) Antibody;抗-GFP（绿色荧光蛋白）抗体store at -20℃，产品报价联系微信jinshanbio